Department of Biochemistry, Afe Babalola University, Ado-Ekiti, Nigeria.
Department of Biochemistry, University of Ilorin, Ilorin, Nigeria.
BMC Complement Altern Med. 2019 Mar 19;19(1):71. doi: 10.1186/s12906-019-2481-0.
Ocimum gratissimum L. is a medicinal plant widely grown in tropical and subtropical regions with the leaf decoction usually taken in folk medicine to enhance erectile performance in men although the probable mechanism of actions remains undetermined. This study examined the inhibitory potentials of Ocimum gratissimum leaves on some key enzymes associated with erectile dysfunction in penile and testicular tissues of the rat.
Inhibitory effect of aqueous extract (1:10 w/v) of O. gratissimum leaves on the activities of phosphodiesterase-5 (PDE-5), arginase, angiotensin I -converting enzyme (ACE), and acetylcholinesterase (AChE) in penile and testicular tissues were assessed. Also, the extract was investigated for ferric reducing antioxidant property(FRAP) and 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging abilities.
The extract showed higher PDE-5 (IC = 43.19 μg/mL), ACE (IC = 44.23 μg/mL), AChE (IC = 55.51 μg/mL) and arginase (IC = 46.12 μg/mL) inhibitory activity in the penile tissue than PDE-5 (IC = 44.67 μg/mL), ACE (IC = 53.99 μg/mL), AChE (IC = 60.03 μg/mL) and arginase (IC = 49.12 μg/mL) inhibitory activity in the testicular tissue homogenate. Furthermore, the extract scavenged free radicals and in a dose-dependent manner.
The enzyme activities displayed might be associated with the bioactive compounds present in the extract which could possibly explain its use in the management of erectile dysfunction (ED).
奥克西米特·格拉蒂西姆 L. 是一种药用植物，广泛生长在热带和亚热带地区，其叶片汤剂通常在民间医学中被用于增强男性的勃起功能，尽管其可能的作用机制仍未确定。本研究检查了奥克西米特·格拉蒂西姆叶片对大鼠阴茎和睾丸组织中与勃起功能障碍相关的一些关键酶的抑制潜力。
评估了奥克西米特·格拉蒂西姆叶片水提取物（1:10w/v）对磷酸二酯酶-5（PDE-5）、精氨酸酶、血管紧张素 I 转化酶（ACE）和乙酰胆碱酯酶（AChE）在阴茎和睾丸组织中的活性的抑制作用。此外，还研究了提取物的铁还原抗氧化能力（FRAP）和 1,1-二苯基-2-苦基肼（DPPH）自由基清除能力。
提取物在阴茎组织中表现出更高的 PDE-5（IC=43.19μg/mL）、ACE（IC=44.23μg/mL）、AChE（IC=55.51μg/mL）和精氨酸酶（IC=46.12μg/mL）抑制活性比 PDE-5（IC=44.67μg/mL）、ACE（IC=53.99μg/mL）、AChE（IC=60.03μg/mL）和精氨酸酶（IC=49.12μg/mL）在睾丸组织匀浆中的抑制活性。此外，提取物以剂量依赖的方式清除自由基。
所显示的酶活性可能与提取物中存在的生物活性化合物有关，这可能解释了其在勃起功能障碍（ED）管理中的应用。
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