Ward Jerrold M, Schofield Paul N, Sundberg John P
Global VetPathology, Montgomery Village, Maryland, USA.
Department of Physiology Development and Neuroscience, University of Cambridge, Downing Street, Cambridge, UK.
Lab Anim (NY). 2017 Mar 22;46(4):146-151. doi: 10.1038/laban.1214.
Reproducibility of in vivo research using the mouse as a model organism depends on many factors, including experimental design, strain or stock, experimental protocols, and methods of data evaluation. Gross and histopathology are often the endpoints of such research and there is increasing concern about the accuracy and reproducibility of diagnoses in the literature. To reproduce histopathological results, the pathology protocol, including necropsy methods and slide preparation, should be followed by interpretation of the slides by a pathologist familiar with reading mouse slides and familiar with the consensus medical nomenclature used in mouse pathology. Likewise, it is important that pathologists are consulted as reviewers of manuscripts where histopathology is a key part of the investigation. The absence of pathology expertise in planning, executing and reviewing in vivo research using mice leads to questionable pathology-based findings and conclusions from studies, even in high-impact journals. We discuss the various aspects of this problem, give some examples from the literature and suggest solutions.
使用小鼠作为模式生物进行体内研究的可重复性取决于许多因素，包括实验设计、品系或种群、实验方案以及数据评估方法。大体病理学和组织病理学往往是此类研究的终点，并且文献中对诊断的准确性和可重复性的关注日益增加。为了重现组织病理学结果，病理方案，包括尸检方法和玻片制备，应由熟悉阅读小鼠玻片并熟悉小鼠病理学中使用的共识医学命名法的病理学家对玻片进行解读。同样，在组织病理学是研究关键部分的稿件评审中咨询病理学家也很重要。在使用小鼠进行体内研究的规划、执行和评审过程中缺乏病理学专业知识，会导致基于病理学的研究结果和结论存在疑问，即使在高影响力期刊上发表的研究也是如此。我们讨论了这个问题的各个方面，从文献中给出了一些例子并提出了解决方案。
