一种使用全细胞抗原检测禽网状内皮组织增殖症病毒抗体的酶联免疫吸附测定法。
An enzyme-linked immunosorbent assay for the detection of antibodies to avian reticuloendotheliosis virus using whole cell antigen.
作者信息
Nicholas R A, Thornton D H
机构信息
Central Veterinary Laboratory, Weybridge, Surrey.
出版信息
Res Vet Sci. 1987 Nov;43(3):403-4.
PMID:2832914
Abstract
An enzyme-linked immunosorbent assay (ELISA) using reticuloendotheliosis virus-infected chick embryo fibroblasts as coating antigen is described for the detection of antibodies to reticuloendotheliosis virus in chicken sera. The ELISA was specific and during the early stages of infection more sensitive than an indirect fluorescent antibody test.
摘要
本文描述了一种酶联免疫吸附测定（ELISA）方法，该方法使用感染网状内皮组织增殖病病毒的鸡胚成纤维细胞作为包被抗原，用于检测鸡血清中网状内皮组织增殖病病毒的抗体。该ELISA具有特异性，且在感染早期比间接荧光抗体试验更灵敏。
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