Todd D, Mackie D P, Mawhinney K A, Connor T J, McNeilly F, McNulty M S
Veterinary Research Laboratories, Stormont, Belfast, Northern Ireland.
Avian Dis. 1990 Apr-Jun;34(2):359-63.
An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to chicken anemia agent (CAA) has been developed. This test utilizes a CAA-specific mouse monoclonal antibody to selectively capture virus antigen. Chicken antibodies to CAA bind to the captured antigen and are detected with horseradish peroxidase-labeled anti-chicken immunoglobulin using a conventional indirect ELISA protocol. When 388 chicken sera from specific-pathogen-free and commercial flocks from the United Kingdom, West Germany, the United States and Australia were examined, 98.5% agreement was obtained between the results of the ELISA and the indirect immunofluorescence assay. This ELISA should have worldwide application in testing SPF and commercial chicken flocks for CAA antibodies.
已开发出一种用于检测鸡贫血因子（CAA）抗体的酶联免疫吸附测定（ELISA）。该检测利用一种CAA特异性小鼠单克隆抗体来选择性捕获病毒抗原。鸡针对CAA的抗体与捕获的抗原结合，并使用传统的间接ELISA方案，通过辣根过氧化物酶标记的抗鸡免疫球蛋白进行检测。当检测来自英国、西德、美国和澳大利亚的无特定病原体鸡群和商业鸡群的388份鸡血清时，ELISA结果与间接免疫荧光测定结果之间的一致性达到98.5%。这种ELISA在检测无特定病原体鸡群和商业鸡群中的CAA抗体方面应具有全球适用性。
