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[绵羊感染牛地方流行性白血病病毒的实验]

[Experimental infection of sheep with the virus of bovine enzootic leukemia].

作者信息

Sandev N

出版信息

Vet Med Nauki. 1987;24(9):31-5.

PMID:2833006
Abstract

The intraperitoneal, the muscular, and the nasal infection with a virus, containing material (blood of a cow, affected by leukosis, with 48100 leucocytes in a cubic mm and 92% of lymphocytosis) and cell suspension FLK, producing leukosis virus, provokes leukosis infection. It was established that the peroral and the intraconjunctival application of virus material does not provoke leukosis till the 6th month (term of observation). With saliva and semen, obtained from positive serologic reagents with persistent lymphocytosis also does not provoke infection. By the immunofluorescent test, in the infected sheep were observed antibodies against the summary glycoprotein antigen, which appear after 1-2 months with continuous seroconversion till 18th month and precipitation titer 1:2 till 1:64. Among 30% of the sheep, after the 5th and 8th month, was established leucocytosis, up to 18800 leucocytes, and persistent lymphocytosis--91-93%.

摘要

用含有病毒的物质(患白血病的母牛血液,每立方毫米含48100个白细胞且淋巴细胞增多症占92%)和产生白血病病毒的细胞悬液进行腹腔内、肌肉内和鼻腔感染,会引发白血病感染。已确定,经口和结膜内应用病毒物质在第6个月(观察期)之前不会引发白血病。从具有持续性淋巴细胞增多症的阳性血清学试剂中获取的唾液和精液也不会引发感染。通过免疫荧光试验,在受感染的绵羊中观察到针对总糖蛋白抗原的抗体,这些抗体在1 - 2个月后出现,血清持续转化直至第18个月,沉淀效价为1:2至1:64。在30%的绵羊中,在第5个月和第8个月后出现白细胞增多,白细胞计数高达18800个,且持续性淋巴细胞增多症占91 - 93%。

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