Patel Dimple M, Fougat Ranbir S, Sakure Amar A, Kumar Sushil, Kumar Mukesh, Mistry Jigar G
Department of Agriculture Biotechnology, Anand Agricultural University, Anand, 388 110, India.
3 Biotech. 2016 Jun;6(1):55. doi: 10.1007/s13205-016-0391-0. Epub 2016 Feb 13.
In the present study, 20 sandalwood (Santalum album L.) genotypes were characterized using RAPD, ISSR and SSR markers. Twenty-five RAPD and twenty-one ISSR primers that generated clear and reproducible banding patterns amplified 225 and 208 bands, respectively, among 20 sandalwood genotypes. Out of 225, 181 (83.13 %) RAPD bands were polymorphic while out of 208, 156 (75.77 %) ISSR bands were polymorphic. The average polymorphism information content (PIC) for RAPD and ISSR was 0.84 and 0.86, respectively. A good correlation (0.96) was observed between the matrices produced by RAPD and ISSR primers. Though, there was high similarity among genotypes (0.79 for RAPD and 0.70 for ISSR), the observed genetic diversity was found good enough for the characterization of sandalwood genotypes. Cross-species transferability SSR markers developed in S. austrocaledonicum and S. insulare were found to be monomorphic. The results of the present investigation would provide valid guidelines for collection, conservation and characterization of sandalwood genetic resources.
在本研究中，利用随机扩增多态性DNA（RAPD）、简单序列重复区间（ISSR）和简单序列重复（SSR）标记对20个檀香（Santalum album L.）基因型进行了特征分析。25个产生清晰且可重复条带模式的RAPD引物和21个ISSR引物，分别在20个檀香基因型中扩增出225条和208条带。在225条RAPD带中，181条（83.13%）具有多态性；在208条ISSR带中，156条（75.77%）具有多态性。RAPD和ISSR的平均多态性信息含量（PIC）分别为0.84和0.86。观察到RAPD和ISSR引物产生的矩阵之间具有良好的相关性（0.96）。虽然基因型之间存在高度相似性（RAPD为0.79，ISSR为0.70），但观察到的遗传多样性足以用于檀香基因型的特征分析。在澳洲檀香（S. austrocaledonicum）和海岛檀香（S. insulare）中开发的跨物种可转移性SSR标记被发现是单态的。本研究结果将为檀香遗传资源的收集、保存和特征分析提供有效的指导方针。
