Mukram Ismailsab, Ramesh Masarbo, Monisha T R, Nayak Anand S, Karegoudar T B
Department of Biochemistry, Gulbarga University, Kalaburagi, 585106, Karnataka, India.
3 Biotech. 2016 Dec;6(2):141. doi: 10.1007/s13205-016-0456-0. Epub 2016 Jun 22.
A nitrile utilizing bacterium Rhodococcus sp. MTB5 was previously isolated in our laboratory by the enrichment culture technique. It is able to utilize butyronitrile as sole carbon, nitrogen, and energy source. Maximum butyronitrile degrading property of this strain has been investigated. Results reveal that 100, 98, and 88 % degradation was achieved for 2, 2.5, and 3 % butyronitrile, respectively. The strain is capable of growing in as high as 5 % butyronitrile concentration. A two-step pathway involving nitrile hydratase (NHase) and amidase was observed for the biodegradation of butyronitrile. Complete degradation (mineralization) of butyronitrile with the help of metabolite feeding experiment was reported. The significance of this investigation was the capability of the strain to completely degrade and its ability to grow on higher concentrations of butyronitrile. These potential features make it a suitable candidate for practical field application for effective in situ bioremediation of butyronitrile contaminated sites.
我们实验室之前通过富集培养技术分离出了一株利用腈的红球菌属MTB5菌株。它能够将丁腈作为唯一的碳源、氮源和能源。已经对该菌株的最大丁腈降解特性进行了研究。结果表明，对于2%、2.5%和3%的丁腈，降解率分别达到了100%、98%和88%。该菌株能够在高达5%的丁腈浓度下生长。观察到丁腈生物降解存在一条涉及腈水合酶（NHase）和酰胺酶的两步途径。通过代谢物添加实验报道了丁腈的完全降解（矿化）。这项研究的意义在于该菌株能够完全降解且能在较高浓度的丁腈上生长。这些潜在特性使其成为丁腈污染场地有效原位生物修复实际现场应用的合适候选菌株。
