单一培养和混合培养对单一酚类化合物和混合酚类化合物利用情况的比较研究。
A comparative study of utilization of single and mixed phenolic compounds by individual and mixed culture.
作者信息
Tyagaturu Renukaprasad Monisha, Ismailsab Mukram, Reddy Pooja V, Baburao Kirankumar, Nayak Anand S, Karegoudar T B
机构信息
Department of Biochemistry, Gulbarga University, Kalaburagi, Karnataka, 585106, India.
出版信息
3 Biotech. 2017 Jul;7(3):215. doi: 10.1007/s13205-017-0815-5. Epub 2017 Jul 1.
Three bacterial strains; Pseudomonas sp. TRMK1, Stenotrophomonas sp. TRMK2 and Xanthomonas sp. TRMK3 were isolated from agro-industrial waste by enrichment culture technique that are capable of utilizing phenolic acids as sole source of carbon and energy. These strains were found to utilize p-coumaric, ferulic and caffeic acid. The individual strains utilized 5 mM of mixed phenolic acids within 20 h of incubation. The bacterial consortium composing these strains was prepared and studied the efficient degradation of phenolic compounds. The bacterial consortium showed the enhanced utilization of 30 mM individual and 25 mM mixed phenolic acids within 32 and 40 h of incubation, respectively. The degradation efficiency of these strains in all the above experiments was above 90%. The prepared bacterial consortium serves as a suitable method for the in situ application of sites contaminated with wide range of phenolic compounds.
通过富集培养技术从农业工业废弃物中分离出三株细菌菌株，分别为假单胞菌属TRMK1、嗜麦芽窄食单胞菌属TRMK2和黄单胞菌属TRMK3，它们能够利用酚酸作为唯一的碳源和能源。发现这些菌株能够利用对香豆酸、阿魏酸和咖啡酸。各菌株在培养20小时内利用5 mM的混合酚酸。制备了由这些菌株组成的细菌联合体，并研究了其对酚类化合物的高效降解。该细菌联合体分别在培养32小时和40小时内对30 mM的单一酚酸和25 mM的混合酚酸表现出增强的利用能力。在上述所有实验中，这些菌株的降解效率均高于90%。所制备的细菌联合体是一种适用于原位处理受多种酚类化合物污染场地的方法。
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