Centre for Microbial Biotechnology, Panjab University, Chandigarh, 160014 India.
Institute of Microbial Technology, Chandigarh, 160036 India.
Indian J Microbiol. 2014 Jun;54(2):232-8. doi: 10.1007/s12088-014-0453-0. Epub 2014 Feb 15.
A nitrile-hydrolysing bacterium, identified as Isoptericola variabilis RGT01, was isolated from industrial effluent through enrichment culture technique using acrylonitrile as the carbon source. Whole cells of this microorganism exhibited a broad range of nitrile-hydrolysing activity as they hydrolysed five aliphatic nitriles (acetonitrile, acrylonitrile, propionitrile, butyronitrile and valeronitrile), two aromatic nitriles (benzonitrile and m-Tolunitrile) and two arylacetonitriles (4-Methoxyphenyl acetonitrile and phenoxyacetonitrile). The nitrile-hydrolysing activity was inducible in nature and acetonitrile proved to be the most efficient inducer. Minimal salt medium supplemented with 50 mM acetonitrile, an incubation temperature of 30 °C with 2 % v/v inoculum, at 200 rpm and incubation of 48 h were found to be the optimal conditions for maximum production (2.64 ± 0.12 U/mg) of nitrile-hydrolysing activity. This activity was stable at 30 °C as it retained around 86 % activity after 4 h at this temperature, but was thermolabile with a half-life of 120 min and 45 min at 40 °C and 50 °C respectively.
一种腈水解细菌，被鉴定为异单胞菌 RGT01，通过使用丙烯腈作为碳源的富集培养技术从工业废水中分离出来。该微生物的完整细胞表现出广泛的腈水解活性，因为它们水解了五种脂肪族腈（乙腈、丙烯腈、丙腈、丁腈和戊腈）、两种芳香族腈（苯腈和间甲苯腈）和两种芳基乙腈（4-甲氧基苯乙腈和苯氧基乙腈）。腈水解活性是诱导性的，乙腈被证明是最有效的诱导剂。在含有 50mM 乙腈的最小盐培养基中，在 30°C 下以 2%v/v 接种量、200rpm 搅拌和 48h 孵育的条件下，发现是最大产腈水解活性（2.64±0.12 U/mg）的最佳条件。该活性在 30°C 下稳定，因为在该温度下 4 小时后保留了约 86%的活性，但在 40°C 和 50°C 下分别具有半衰期为 120 分钟和 45 分钟的热不稳定性。
