Nawaz M S, Heinze T M, Cerniglia C E
National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079.
Appl Environ Microbiol. 1992 Jan;58(1):27-31. doi: 10.1128/aem.58.1.27-31.1992.
A strain of Klebsiella pneumoniae that used aliphatic nitriles as the sole source of nitrogen was adapted to benzonitrile as the sole source of carbon and nitrogen. Gas chromatographic and mass spectral analyses of culture filtrates indicated that K. pneumoniae metabolized 8.4 mM benzonitrile to 4.0 mM benzoic acid and 2.7 mM ammonia. In addition, butyronitrile was metabolized to butyramide and ammonia. The isolate also degraded mixtures of benzonitrile and aliphatic nitriles. Cell extracts contained nitrile hydratase and amidase activities. The enzyme activities were higher with butyronitrile and butyramide than with benzonitrile and benzamide, and amidase activities were twofold higher than nitrile hydratase activities. K. pneumoniae appears promising for the bioremediation of sites contaminated with aliphatic and aromatic nitriles.
一株以脂肪族腈作为唯一氮源的肺炎克雷伯菌被驯化，使其能够利用苯甲腈作为唯一的碳源和氮源。对培养滤液进行气相色谱和质谱分析表明，肺炎克雷伯菌将8.4 mM苯甲腈代谢为4.0 mM苯甲酸和2.7 mM氨。此外，丁腈被代谢为丁酰胺和氨。该分离株还能降解苯甲腈和脂肪族腈的混合物。细胞提取物含有腈水合酶和酰胺酶活性。丁腈和丁酰胺的酶活性高于苯甲腈和苯甲酰胺，且酰胺酶活性比腈水合酶活性高两倍。肺炎克雷伯菌在被脂肪族和芳香族腈污染的场地的生物修复方面似乎很有前景。
