Kumari Vijaya, Kumar Vijay, Chauhan Ravin, Asif Mohammad, Bhalla Tek Chand
Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, 171005, Himachal Pradesh, India.
3 Biotech. 2016 Dec;6(2):149. doi: 10.1007/s13205-016-0460-4. Epub 2016 Jun 29.
Cutinases are hydrolytic enzymes which catalyzes esterification and trans-esterification reactions that make them highly potential industrial biocatalyst. In the present investigation microorganisms showing cutinase activity were isolated from plant samples. The strain showing maximum cutinase activity was identified by 18S rDNA sequencing as Aspergillus sp. RL2Ct and was selected for further studies. To achieve maximum enzyme production, the medium components affecting cutinase production were screened by Plackett-Burman followed by central composite design. The results obtained suggested that cutin, temperature and CaCl have influenced the cutinase production significantly with very high confidence levels. Cutinase production was maximum (663 U/mg protein) when using cutin prepared from orange peel as sole source of carbon. An overall 4.33-fold increase in the production of cutinase was observed after optimization of culture conditions (including 2.5-fold increase using RSM) during 24 h of incubation. The production time of Aspergillus sp. RL2Ct cutinase is significantly lower than the most of the earlier reported cutinase-producing fungus.
角质酶是一种水解酶，可催化酯化和转酯化反应，这使得它们成为极具潜力的工业生物催化剂。在本研究中，从植物样本中分离出了具有角质酶活性的微生物。通过18S rDNA测序鉴定出表现出最高角质酶活性的菌株为曲霉属RL2Ct，并选择其进行进一步研究。为了实现最大酶产量，通过Plackett-Burman设计筛选影响角质酶产生的培养基成分，随后进行中心复合设计。获得的结果表明，角质、温度和氯化钙对角质酶的产生有显著影响，置信水平非常高。当使用由橙皮制备的角质作为唯一碳源时，角质酶产量最高（663 U/mg蛋白质）。在24小时培养期间优化培养条件（包括使用响应面法提高2.5倍）后，观察到角质酶产量总体增加了4.33倍。曲霉属RL2Ct角质酶的生产时间明显低于大多数早期报道的产角质酶真菌。
