作为细胞保护超氧自由基清除剂的膳食类黄酮铁络合物。

Dietary flavonoid iron complexes as cytoprotective superoxide radical scavengers.

作者信息

Moridani Majid Y, Pourahmad Jalal, Bui Hoang, Siraki Arno, O'Brien Peter J

机构信息

Faculty of Pharmacy, University of Toronto, Toronto, Ontario, Canada.

出版信息

Free Radic Biol Med. 2003 Jan 15;34(2):243-53. doi: 10.1016/s0891-5849(02)01241-8.

DOI:10.1016/s0891-5849(02)01241-8

PMID:12521606

Abstract

Superoxide radicals have been implicated in the pathogenesis of ischemia/reperfusion, aging, and inflammatory diseases. In the present work, we have shown that the Fe(3+) complexes of flavonoids (polyphenols) were much more effective than the uncomplexed flavonoids in protecting isolated rat hepatocytes against hypoxia-reoxygenation injury. The 2:1 flavonoid-metal complexes of Cu(2+), Fe(2+), or Fe(3+) were more effective than the parent compounds in scavenging superoxide radicals generated by xanthine oxidase/hypoxanthine (an enzymatic superoxide-generating system). The 2:1 [flavonoid:Fe(3+)] complexes but not the [deferoxamine:Fe(3+)] complex readily scavenged superoxide radicals. These results suggest that the initial step in superoxide radical scavenging (SRS) activity involves a redox-active flavonoid:Fe(3+) complex. Flavonoid:Fe(3+) complexes should, therefore, be tested as a therapy for the treatment of ischemia/reperfusion injury.

摘要

超氧自由基与缺血/再灌注、衰老及炎症性疾病的发病机制有关。在本研究中，我们发现黄酮类化合物（多酚）的Fe(3+)络合物在保护离体大鼠肝细胞免受缺氧-复氧损伤方面比未络合的黄酮类化合物有效得多。Cu(2+)、Fe(2+)或Fe(3+)的2:1黄酮类金属络合物在清除黄嘌呤氧化酶/次黄嘌呤（一种酶促超氧生成系统）产生的超氧自由基方面比母体化合物更有效。2:1 [黄酮类化合物:Fe(3+)]络合物而非[去铁胺:Fe(3+)]络合物能迅速清除超氧自由基。这些结果表明，超氧自由基清除（SRS）活性的初始步骤涉及具有氧化还原活性的黄酮类化合物:Fe(3+)络合物。因此，黄酮类化合物:Fe(3+)络合物应作为治疗缺血/再灌注损伤的一种疗法进行测试。