STROMALab (Team 2), Université de Toulouse, EFS, INP-ENVT, INSERM U1031, UPS, 31042 Toulouse, France; EFS Pyrénées-Méditerranée, 31042 Toulouse, France.
STROMALab (Team 1), Université de Toulouse, CNRS ERL5311, EFS, INP-ENVT, INSERM U1031, UPS, 31042 Toulouse, France.
Stem Cell Reports. 2017 Apr 11;8(4):961-976. doi: 10.1016/j.stemcr.2017.02.008. Epub 2017 Mar 16.
Mesenchymal stromal cells (MSCs) sense and modulate inflammation and represent potential clinical treatment for immune disorders. However, many details of the bidirectional interaction of MSCs and the innate immune compartment are still unsolved. Here we describe an unconventional but functional interaction between pro-inflammatory classically activated macrophages (M1MΦ) and MSCs, with CD54 playing a central role. CD54 was upregulated and enriched specifically at the contact area between M1MФ and MSCs. Moreover, the specific interaction induced calcium signaling and increased the immunosuppressive capacities of MSCs dependent on CD54 mediation. Our data demonstrate that MSCs can detect an inflammatory microenvironment via a direct and physical interaction with innate immune cells. This finding opens different perspectives for MSC-based cell therapy.
间充质基质细胞（MSCs）能够感知和调节炎症，代表了治疗免疫紊乱的潜在临床治疗方法。然而，MSCs 与先天免疫区室的双向相互作用的许多细节仍未解决。在这里，我们描述了促炎经典激活的巨噬细胞（M1MΦ）和 MSCs 之间一种非传统但功能上的相互作用，其中 CD54 起着核心作用。CD54 在上调并在 M1MФ 和 MSCs 之间的接触区域富集。此外，这种特异性相互作用诱导钙信号，并增加 MSC 的免疫抑制能力，这取决于 CD54 的介导。我们的数据表明，MSCs 可以通过与先天免疫细胞的直接物理相互作用来检测炎症微环境。这一发现为基于 MSC 的细胞治疗开辟了不同的视角。
