Wang Qiuxia, Ou Changbo, Dou Yongxi, Chen Lei, Meng Xuelian, Liu Xingyou, Yu Yan, Jiang Jinqing, Ma Jinyou, Zhang Zhidong, Hu Jianhe, Cai Xuepeng
Henan Institute of Science and Technology, Xinxiang 453003, Henan, China; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Henan Institute of Science and Technology, Xinxiang 453003, Henan, China.
Microb Pathog. 2017 Jun;107:81-87. doi: 10.1016/j.micpath.2017.03.021. Epub 2017 Mar 19.
Peste des petits ruminants virus (PPRV), belonging to paramyxoviruses, has six structure proteins (such as matrix protein (M), nucleocapsid proteins (N), fusion protein (F) and hemagglutinin protein (H)) and could cause high morbidity and mortality in sheep and goats. Although a vaccine strain of PPRV has been rescued and co-expression of M and N could yield PPRV-like particles, the roles of structure proteins in virion assembly and release have not been investigated in detail. In this study, plasmids carrying PPRV cDNA sequences encoding the N, M, H, and F proteins were expressed in Vero cells. The co-expression of all four proteins resulted in the release of virus-like particles (VLPs) with similar release efficiency to that of authentic virions. Moreover, the co-expression of M together with F also resulted in efficient VLPs release. In the absence of M protein, the expression of no combination of the other proteins resulted in particle release. In summary, a VLPs production system for PPRV has been established and M protein is necessary for promoting the assembly and release of VLPs, of which the predominant protein is M protein. Further study will be focused on the immunogenicity of the VLPs.
小反刍兽疫病毒（PPRV）属于副粘病毒，有六种结构蛋白（如基质蛋白（M）、核衣壳蛋白（N）、融合蛋白（F）和血凝素蛋白（H）），可在绵羊和山羊中引起高发病率和高死亡率。尽管已拯救出PPRV疫苗株，且M和N的共表达可产生类似PPRV的颗粒，但结构蛋白在病毒体组装和释放中的作用尚未得到详细研究。在本研究中，携带编码N、M、H和F蛋白的PPRV cDNA序列的质粒在Vero细胞中表达。这四种蛋白的共表达导致病毒样颗粒（VLP）的释放，其释放效率与真实病毒体相似。此外，M与F的共表达也导致VLP的高效释放。在没有M蛋白的情况下，其他蛋白的任何组合表达都不会导致颗粒释放。总之，已建立了PPRV的VLP生产系统，M蛋白是促进VLP组装和释放所必需的，其中主要蛋白是M蛋白。进一步的研究将集中在VLP的免疫原性上。
