Oguma Keisuke, Suzuki Miho, Sentsui Hiroshi
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan.
Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan.
Virus Res. 2017 Apr 2;233:120-124. doi: 10.1016/j.virusres.2017.03.016. Epub 2017 Mar 19.
A two-month-old calf was diagnosed with leukosis on the basis of the clinical sign of enlarged, superficial lymph nodes. Serological and genetic tests for bovine leukemia virus (BLV) were performed because the calf was born from a cow infected with BLV. The serum had a weakly positive BLV antibody, and the BLV provirus was detected within neoplastic cells on performing polymerase chain reaction (PCR). Analysis of the BLV provirus integration site using inverse PCR revealed that the BLV integration site location was identical on all chromosomes in all tumor tissues examined. Thus, the tumor cells monoclonally proliferated following BLV infection. The present study shows that enzootic bovine leukosis can occur in a young animal, as in the two-month-old calf in our study.
根据浅表淋巴结肿大的临床症状，一头两个月大的小牛被诊断为白血病。由于这头小牛是由感染牛白血病病毒（BLV）的母牛所生，因此对其进行了牛白血病病毒的血清学和基因检测。血清中BLV抗体呈弱阳性，通过聚合酶链反应（PCR）在肿瘤细胞中检测到了BLV前病毒。使用反向PCR分析BLV前病毒整合位点发现，在所检查的所有肿瘤组织中，所有染色体上的BLV整合位点位置均相同。因此，肿瘤细胞在感染BLV后发生单克隆增殖。本研究表明，地方流行性牛白血病可发生于幼龄动物，如我们研究中的两个月大小牛。
