Gouveia Leonor, Betsholtz Christer, Andrae Johanna
Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden.
Integrated Cardio Metabolic Centre, Karolinska Institute, Huddinge, Sweden.
Physiol Rep. 2017 Mar;5(6). doi: 10.14814/phy2.13092.
Activation of the platelet-derived growth factor receptor- (PDGFR) signaling pathway is critically important during lung alveogenesis, the process in lung development during which alveoli are formed from the terminal alveolar sacs. Several studies have aimed to characterize the expression patterns of PDGFR and its two ligands (PDGF-A and -C) in the lung, but published analyses have been limited to embryonic and/or perinatal time points, and no attempts have been made to characterize both receptor and ligand expression simultaneously. In this study, we present a detailed map of the expression patterns of PDGFR, PDGF-A and PDGF-C during the entire period of lung development, that is, from early embryogenesis until adulthood. Three different reporter mice were analyzed ( , , and ), in which either or were expressed under the respective promoter in gene-targeted alleles. A spatiotemporal dynamic expression was identified for both ligands and receptor. PDGF-A and PDGF-C were located to distinct populations of epithelial and smooth muscle cells, whereas PDGFR expression was located to different mesenchymal cell populations. The detailed characterization of gene expression provides a comprehensive map of PDGFR signaling in lung cells, opening up for a better understanding of the role of PDGF signaling during lung development.
血小板衍生生长因子受体 - （PDGFR）信号通路的激活在肺腺泡形成过程中至关重要，肺腺泡形成是肺发育过程中由终末肺泡囊形成肺泡的过程。多项研究旨在表征PDGFR及其两种配体（PDGF - A和 - C）在肺中的表达模式，但已发表的分析仅限于胚胎期和/或围产期时间点，且尚未有人尝试同时表征受体和配体的表达。在本研究中，我们展示了肺发育全过程，即从早期胚胎发生到成年期，PDGFR、PDGF - A和PDGF - C表达模式的详细图谱。分析了三种不同的报告基因小鼠（ 、 和 ），其中 或 在基因靶向等位基因的各自启动子下表达。确定了配体和受体的时空动态表达。PDGF - A和PDGF - C定位于不同的上皮细胞和平滑肌细胞群体，而PDGFR表达定位于不同的间充质细胞群体。基因表达的详细表征提供了肺细胞中PDGFR信号传导的全面图谱，有助于更好地理解PDGF信号在肺发育过程中的作用。
