Stumpf W E, Clark S A, O'Brien L P, Reid F A
Department of Anatomy, University of North Carolina, Chapel Hill 27514.
Anat Embryol (Berl). 1988;177(4):307-10. doi: 10.1007/BF00315837.
Autoradiographic studies revealed concentration of 3H 1,25(OH)2 vitamin D3 in nuclei of certain neurons in the spinal cord of adult and neonatal mice, fed a normal or a vitamin D deficient diet. Nuclear uptake and retention was strongest in motor neurons in lamina IX. Nuclear concentration also existed in neurons of lamina II, lamina VIII, lamina X and intermediate nucleus of the lateral column. The results indicate that these neurons are target neurons which contain nuclear receptors for 1,25(OH)2 vitamin D3. This suggests that 1,25(OH)2 vitamin D3 has direct genomic actions on the innervation of skeletal muscle by exerting related trophic, secretory, and electrophysiological effects. In addition, these data point to direct genomic actions of 1,25(OH)2 vitamin D3 on spinal sensory perception, and on certain autonomic functions. Nuclear binding in certain neurons in the peripheral ganglion of the trigeminal nerve further suggests that sensory perception is influenced by 1,25(OH)2 vitamin D3 not only at the level of the substantia gelatinosa, but also at the level of spinal ganglia.
放射自显影研究显示，在喂食正常或维生素D缺乏饮食的成年和新生小鼠脊髓中，某些神经元的细胞核内有3H 1,25(OH)2维生素D3的聚集。在第IX层的运动神经元中，核摄取和保留最强。在第II层、第VIII层、第X层和外侧柱中间核的神经元中也存在核聚集。结果表明，这些神经元是含有1,25(OH)2维生素D3核受体的靶神经元。这表明1,25(OH)2维生素D3通过发挥相关的营养、分泌和电生理作用，对骨骼肌的神经支配具有直接的基因组作用。此外，这些数据表明1,25(OH)2维生素D3对脊髓感觉感知和某些自主功能具有直接的基因组作用。三叉神经外周神经节中某些神经元的核结合进一步表明，1,25(OH)2维生素D3不仅在胶状质水平，而且在脊髓神经节水平影响感觉感知。
