Pucci M J, Monteschio M E, Kemker C L
Microlife Technics, Sarasota, Florida 34230.
Appl Environ Microbiol. 1988 Feb;54(2):281-7. doi: 10.1128/aem.54.2.281-287.1988.
Transfer of the broad-host-range resistance plasmids pIP501 and pAM beta 1 from Streptococcus faecalis to Leuconostoc dextranicum and Leuconostoc cremoris occurred between cells that were immobilized on nitrocellulose filters in the presence of DNase. Transfer of pIP501 to Leuconostoc spp. also occurred when Streptococcus sanguis and Streptococcus lactis were used as donors. In addition, transfer of pIP501 and pAM beta 1 was observed from L. cremoris and L. dextranicum transconjugants to S. sanguis and S. faecalis. Expression of the pAM beta 1 erythromycin and pIP501 erythromycin and chloramphenicol resistance determinants was essentially equivalent in donors and transconjugants. Frequencies of transfer generally ranged from 10(-4) to 10(-7) transconjugants per input donor cell. Intrageneric transfer of pIP501 and pAM beta 1 occurred between L. cremoris and L. dextranicum strains in the same approximate range. These data further extend the host range of pIP501 and pAM beta 1 and demonstrate another example of gene transfer in the genus Leuconostoc.
在存在脱氧核糖核酸酶的情况下，广泛宿主范围的抗性质粒pIP501和pAMβ1从粪肠球菌转移至右旋糖酐明串珠菌和乳明串珠菌，这种转移发生在固定于硝酸纤维素滤膜上的细胞之间。当血链球菌和乳酸链球菌作为供体时，pIP501也会转移至明串珠菌属。此外，还观察到pIP501和pAMβ1从乳明串珠菌和右旋糖酐明串珠菌转接合子转移至血链球菌和粪肠球菌。pAMβ1红霉素抗性决定簇以及pIP501红霉素和氯霉素抗性决定簇在供体和转接合子中的表达基本相当。转移频率通常为每输入一个供体细胞产生10^(-4)至10^(-7)个转接合子。pIP501和pAMβ1在乳明串珠菌和右旋糖酐明串珠菌菌株之间进行的属内转移频率大致相同。这些数据进一步扩展了pIP501和pAMβ1的宿主范围，并证明了明串珠菌属中基因转移的另一个实例。
