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补体结合试验、免疫荧光试验及免疫酶试验用于巨细胞病毒IgG抗体常规检测的比较

Comparison of complement fixation, immunofluorescence and immunoenzymatic tests for routine detection of cytomegalovirus IgG antibodies.

作者信息

Ammendolia L, Andreoni M, Tosco P, Volpi A

出版信息

Boll Ist Sieroter Milan. 1984 Jul 31;63(3):216-8.

PMID:6095883
Abstract

Complement fixation (CF), immunofluorescence (IF) and immunoenzymatic (ELISA) tests by commercial sources were compared, for routine laboratory detection of cytomegalovirus IgG antibodies, on 120 serum samples from normal pregnant females. IF and ELISA were comparable in terms of specificity and sensitivity, but in our opinion ELISA showed some advantages with regard to need for facilities and trained personnel. CF could not detect some of low titer positive sera, so it can not be suggested where it is critical to know a previous contact with the virus, as in blood donors or transplant donors and recipients.

摘要

对来自正常怀孕女性的120份血清样本进行了比较,以通过商业来源的补体结合(CF)、免疫荧光(IF)和免疫酶法(ELISA)检测,用于巨细胞病毒IgG抗体的常规实验室检测。IF和ELISA在特异性和敏感性方面相当,但我们认为ELISA在设施和训练有素的人员需求方面具有一些优势。CF无法检测到一些低滴度阳性血清,因此在了解先前是否接触过该病毒至关重要的情况下,如在献血者或移植供体及受者中,不建议使用CF。

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