Landini M P, Mirolo G, Coppolecchia P, Re M C, La Placa M
Microbiol Immunol. 1986;30(7):683-95. doi: 10.1111/j.1348-0421.1986.tb02994.x.
In a longitudinal study we examined by immunoblotting (IB) the development and the evolution of the humoral immune response against individual cytomegalovirus (CMV) structural polypeptides in a total of 80 serum samples from 13 renal transplant recipients showing serological evidence of CMV infection and five renal transplant recipients with an anti-CMV antibody level unchanged over the observation period. The results showed that the IB reactivity at the time of transplantation may be a good index of the host's humoral immune status against CMV; by using this procedure it is possible to identify a seroconversion by the detection of antibodies reacting with some intermediate molecular weight proteins in sera examined at high dilution. Furthermore, IB is a very sensitive procedure also for IgM detection as it anticipates the positivity of the enzyme immune assay for IgM.
在一项纵向研究中，我们通过免疫印迹法（IB）检测了13名有巨细胞病毒（CMV）感染血清学证据的肾移植受者以及5名在观察期内抗CMV抗体水平未变的肾移植受者共80份血清样本中，针对单个CMV结构多肽的体液免疫反应的发展和演变。结果表明，移植时的IB反应性可能是宿主针对CMV的体液免疫状态的良好指标；通过该方法，通过检测与高稀释度检测的血清中一些中等分子量蛋白质发生反应的抗体，可以识别血清转化。此外，IB对于IgM检测也是一种非常灵敏的方法，因为它比IgM的酶免疫测定更早出现阳性。
