MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
Plant Biology Department, Michigan State University, East Lansing, MI 48824, USA.
J Integr Plant Biol. 2017 Jul;59(7):454-458. doi: 10.1111/jipb.12542. Epub 2017 Jun 6.
Peroxisomes are ubiquitous and dynamic eukaryotic organelles capable of altering their abundance in response to environmental and developmental cues, yet the regulatory mechanism of plant peroxisome division/proliferation is unclear. To identify transcriptional regulators of the peroxisome division factor gene PEX11b, we performed a nuclear pull-down experiment and identified Arabidopsis Forkhead-Associated Domain Protein 3 (FHA3) as a novel protein that binds to the promoter of PEX11b. Our data supported the conclusion that, in contrast to the previously identified HY5 HOMOLOG (HYH) protein that promotes the transcription of PEX11b, FHA3 is a negative regulator of PEX11b expression and peroxisome division.
过氧化物酶体是普遍存在且动态的真核细胞器，能够响应环境和发育线索改变其丰度，但植物过氧化物酶体分裂/增殖的调节机制尚不清楚。为了鉴定过氧化物酶体分裂因子基因 PEX11b 的转录调控因子，我们进行了核下拉实验，鉴定出拟南芥 Forkhead-Associated Domain Protein 3（FHA3）是一种与 PEX11b 启动子结合的新型蛋白。我们的数据支持以下结论：与先前鉴定的促进 PEX11b 转录的 HY5 HOMOLOG（HYH）蛋白相反，FHA3 是 PEX11b 表达和过氧化物酶体分裂的负调控因子。
