Aryantini Ni Putu Desy, Kondoh Daisuke, Nishiyama Keita, Yamamoto Yuji, Mukai Takao, Sujaya I Nengah, Urashima Tadasu, Fukuda Kenji
Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
FEMS Microbiol Lett. 2017 Mar 1;364(6). doi: 10.1093/femsle/fnx056.
Anchorless cell surface proteins (CSPs) were extracted with 1 M lithium chloride solution from Lactobacillus rhamnosus FSMM22. Loss of the anchorless CSPs resulted in a 2-fold decrease in FSMM22 cells bound to a constitutive extracellular matrix glycoprotein, laminin, in vitro. DNA-binding protein HU, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and 30S ribosomal protein S19 (RpsS) were identified by mass spectrometry in the extract as laminin-binding adhesins. Among the four proteins, RpsS was immunohistochemically confirmed to exist on the cell surface. Our findings strongly suggest that anchorless CSPs can enhance bacterial adhesion to the host.
用1M氯化锂溶液从鼠李糖乳杆菌FSMM22中提取无锚定细胞表面蛋白（CSPs）。无锚定CSPs的缺失导致FSMM22细胞在体外与组成型细胞外基质糖蛋白层粘连蛋白结合的能力下降了2倍。通过质谱分析在提取物中鉴定出DNA结合蛋白HU、甘油醛-3-磷酸脱氢酶、乳酸脱氢酶和30S核糖体蛋白S19（RpsS）作为层粘连蛋白结合黏附素。在这四种蛋白中，RpsS经免疫组织化学证实存在于细胞表面。我们的研究结果有力地表明，无锚定CSPs可以增强细菌对宿主的黏附。
