Gersch Robert P, Glahn Joshua, Tecce Michael G, Wilson Anthony J, Percec Ivona
From the Department of Surgery, University of Pennsylvania, Philadelphia, PA.
Aesthet Surg J. 2017 Jun 1;37(6):723-729. doi: 10.1093/asj/sjw235.
Adipose-derived stem cells (ASCs) are a powerful tool for cosmetic surgery and regenerative medicine. The use of autologous platelet rich plasma (PRP), particularly in combination with ASC-based therapy, has significantly expanded in recent years. Unfortunately, the mechanisms and optimal dosing responsible for the beneficial effects of PRP remain poorly understood. Here we investigate the effect of PRP on ASC growth and differentiation.
To assess the impact of different PRP feeding and cryopreservation protocols on ASC isolation, expansion, and differentiation.
Human PRP was isolated using the Magellan System (Arteriocyte). Fresh PRP (fPRP), flash frozen PRP (ffPRP), and cryopreserved PRP (cPRP) were added to human ASCs isolated from healthy patients. A panel of PRP supplementation protocols was analyzed for ASC adherence, proliferation, and osteogenesis.
The fresh and cryopreserved PRP groups demonstrated reduced cell adherence compared to control (non-PRP) groups (P < 0.001), while the flash frozen PRP groups showed cell adherence equivalent to or better than controls. After 7 days of growth, ASC populations for fPRP and ffPRP Single Administration protocols were significantly higher than other feeding protocols and controls. This benefit was lost in cPRP groups. Optimized ffPRP protocols showed potential for spontaneous osteogenesis.
Addition of ffPRP improves initial ASC adherence while a single administration of either fresh or flash frozen PRP without additional cell manipulation significantly augments subsequent ASC proliferation. The potential for spontaneous osteogenic differentiation upon PRP exposure invokes the need for additional molecular studies of PRP activity prior to further expansion to clinical applications.
脂肪来源干细胞（ASC）是整形手术和再生医学的有力工具。近年来，自体富血小板血浆（PRP）的应用显著增加，尤其是与基于ASC的治疗联合使用时。不幸的是，PRP产生有益效果的机制和最佳剂量仍知之甚少。在此，我们研究PRP对ASC生长和分化的影响。
评估不同的PRP添加和冷冻保存方案对ASC分离、扩增和分化的影响。
使用麦哲伦系统（Arteriocyte）分离人PRP。将新鲜PRP（fPRP）、快速冷冻PRP（ffPRP）和冷冻保存PRP（cPRP）添加到从健康患者分离的人ASC中。分析一组PRP补充方案对ASC黏附、增殖和成骨的影响。
与对照组（非PRP）相比，新鲜和冷冻保存PRP组的细胞黏附减少（P < 0.001），而快速冷冻PRP组的细胞黏附与对照组相当或更好。生长7天后，fPRP和ffPRP单次给药方案的ASC群体显著高于其他添加方案和对照组。cPRP组则失去了这种优势。优化的ffPRP方案显示出自发成骨的潜力。
添加ffPRP可改善ASC的初始黏附，而单次给予新鲜或快速冷冻PRP且无需额外细胞操作可显著增强随后的ASC增殖。PRP暴露后自发成骨分化的潜力表明，在进一步扩展至临床应用之前，需要对PRP活性进行更多分子研究。
