Department of Biotechnology and Animal Science, National Ilan University, Yilan, Taiwan.
J Food Drug Anal. 2018 Apr;26(2):761-768. doi: 10.1016/j.jfda.2017.10.002. Epub 2017 Nov 10.
Taiwanese green propolis is a prenylated flavonoid rich honeybee product and propolins isolated from Taiwanese green propolis exert a broad spectrum of biological activities, such as anti-cancer and anti-oxidant. However, the anti-bacterial effects of Taiwanese green propolis or propolins are still poorly understood. In the current study, the antibacterial effects of Taiwanese green propolis and propolins were evaluated. Results show that the maximum dry matter yields of Taiwanese green propolis were observed in the 95% and 99.5% ethanol extracts compared to other extraction methods. Consistently, the highest concentration of propolins C, D, F and G from Taiwanese green propolis was obtained in 95% and 99.5% ethanol extracts. Propolins inhibited the growth of gram-positive bacterial strains (Staphylococcus aureus, Bacillus subtilis, Listeria monocytogenes and Paenibacillus larvae). The average minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of propolins from ethanol extracts were 20 μg/ml. Among the propolins, propolin C had the highest antibacterial activity. Furthermore, Taiwanese green propolis also showed antibacterial activity against methicillin-resistant S. aureus (MRSA). In conclusion, these results demonstrate that Taiwanese green propolis and propolins have significant antibacterial activity, particularly against gram-positive bacterial strains.
台湾绿蜂胶是一种富含苯丙素的蜂胶产品，从台湾绿蜂胶中分离得到的普洛马林具有广泛的生物活性，如抗癌和抗氧化作用。然而，台湾绿蜂胶或普洛马林的抗菌作用仍知之甚少。在本研究中，评估了台湾绿蜂胶和普洛马林的抗菌作用。结果表明，与其他提取方法相比，台湾绿蜂胶在 95%和 99.5%乙醇提取物中的最大干物质产量最高。同样，从台湾绿蜂胶中获得的普洛马林 C、D、F 和 G 的最高浓度也出现在 95%和 99.5%乙醇提取物中。普洛马林抑制革兰氏阳性菌（金黄色葡萄球菌、枯草芽孢杆菌、单核细胞增生李斯特菌和蜡状芽孢杆菌）的生长。普洛马林乙醇提取物的平均最小抑菌浓度（MIC）和最小杀菌浓度（MBC）为 20μg/ml。在这些普洛马林中，普洛马林 C 具有最高的抗菌活性。此外，台湾绿蜂胶对耐甲氧西林金黄色葡萄球菌（MRSA）也具有抗菌活性。总之，这些结果表明，台湾绿蜂胶和普洛马林具有显著的抗菌活性，特别是对革兰氏阳性菌。
