Li Xuan, Pang Ka Yan, Ng Tsz Wing, Leung Ping Chung, Zhang Cheng Fei, Leung Ken Cham-Fai, Jin Lijian
Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
Department of Chemistry, Institute of Creativity, and Partner State Key Laboratory of Environmental & Biological Analysis, The Hong Kong Baptist University, Kowloon, Hong Kong, China.
Nanomaterials (Basel). 2016 Oct 27;6(11):192. doi: 10.3390/nano6110192.
Oral mucosa as the front-line barrier in the mouth is constantly exposed to a complex microenvironment with multitudinous microbes. In this study, the interactions of mesoporous silica nanoparticles (MSNs) with primary human gingival epithelial cells were analyzed for up to 72 h, and their diffusion capacity in the reconstructed human gingival epithelia (RHGE) and porcine ear skin models was further assessed at 24 h. It was found that the synthesized fluorescent mesoporous silica nanoparticles (RITC-NPs) with low cytotoxicity could be uptaken, degraded, and/or excreted by the human gingival epithelial cells. Moreover, the RITC-NPs penetrated into the stratum corneum of RHGE in a time-dependent manner, while they were unable to get across the barrier of stratum corneum in the porcine ear skins. Consequently, the penetration and accumulation of RITC-NPs at the corneum layers of epithelia could form a "nanocoating-like barrier". This preliminary proof-of-concept study suggests the feasibility of developing nanoparticle-based antimicrobial and anti-inflammatory agents through topical application for oral healthcare.
口腔黏膜作为口腔的一线屏障，持续暴露于含有众多微生物的复杂微环境中。在本研究中，分析了介孔二氧化硅纳米颗粒（MSNs）与原代人牙龈上皮细胞长达72小时的相互作用，并在24小时时进一步评估了它们在重建人牙龈上皮（RHGE）和猪耳皮肤模型中的扩散能力。结果发现，具有低细胞毒性的合成荧光介孔二氧化硅纳米颗粒（RITC-NPs）可被人牙龈上皮细胞摄取、降解和/或排泄。此外，RITC-NPs以时间依赖性方式渗透到RHGE的角质层中，而它们无法穿过猪耳皮肤的角质层屏障。因此，RITC-NPs在上皮角质层的渗透和积累可形成“纳米涂层样屏障”。这项初步的概念验证研究表明，通过局部应用开发基于纳米颗粒的抗菌和抗炎剂用于口腔保健具有可行性。
