Rahman Masudur, Neff David, Green Nathaniel, Norton Michael L
Parabon Nanolabs, Huntington, WV 25701, USA.
Department of Chemistry, Marshall University, Huntington, WV 25755, USA.
Nanomaterials (Basel). 2016 Oct 31;6(11):196. doi: 10.3390/nano6110196.
Although there is a long history of the study of the interaction of DNA with carbon surfaces, limited information exists regarding the interaction of complex DNA-based nanostructures with the important material graphite, which is closely related to graphene. In view of the capacity of DNA to direct the assembly of proteins and optical and electronic nanoparticles, the potential for combining DNA-based materials with graphite, which is an ultra-flat, conductive carbon substrate, requires evaluation. A series of imaging studies utilizing Atomic Force Microscopy has been applied in order to provide a unified picture of this important interaction of structured DNA and graphite. For the test structure examined, we observe a rapid destabilization of the complex DNA origami structure, consistent with a strong interaction of single-stranded DNA with the carbon surface. This destabilizing interaction can be obscured by an intentional or unintentional primary intervening layer of single-stranded DNA. Because the interaction of origami with graphite is not completely dissociative, and because the frustrated, expanded structure is relatively stable over time in solution, it is demonstrated that organized structures of pairs of the model protein streptavidin can be produced on carbon surfaces using DNA origami as the directing material.
尽管对DNA与碳表面相互作用的研究历史悠久，但关于基于DNA的复杂纳米结构与重要材料石墨（与石墨烯密切相关）之间的相互作用，现有信息有限。鉴于DNA具有指导蛋白质以及光学和电子纳米颗粒组装的能力，将基于DNA的材料与超扁平导电碳基底石墨相结合的潜力需要评估。一系列利用原子力显微镜的成像研究已被应用，以便对结构化DNA与石墨之间的这种重要相互作用提供一个统一的认识。对于所研究的测试结构，我们观察到复杂的DNA折纸结构迅速失稳，这与单链DNA与碳表面的强烈相互作用一致。这种失稳相互作用可能会被单链DNA有意或无意形成的初级中间层掩盖。由于折纸与石墨的相互作用并非完全解离，且由于受挫的、扩展的结构在溶液中随时间相对稳定，因此证明了使用DNA折纸作为导向材料，可以在碳表面产生模型蛋白链霉亲和素的成对有序结构。
