Ramakrishnan Saminathan, Grundmeier Guido, Keller Adrian
Technical and Macromolecular Chemistry, Paderborn University, Paderborn, Germany.
Methods Mol Biol. 2018;1811:253-262. doi: 10.1007/978-1-4939-8582-1_17.
The DNA origami technique has made its way into various areas of nanotechnology, materials science, biophysics, and medicine. Among the many applications of DNA origami nanostructures, their use as masks for patterning of organic and inorganic materials by molecular lithography has received great attention. Here, we describe a protocol for the self-assembly of ordered monolayers of DNA origami nanostructures on mica surfaces and the subsequent fabrication of regular protein patterns over large surface areas via directed adsorption through the DNA origami mask. While the geometry of the pattern is determined by the shape of the DNA origami nanostructures, protein coverage inside the holes of the mask can be varied from single proteins to dense monolayers by adjusting the protein concentration and cationic strength of the adsorption buffer.
DNA折纸技术已进入纳米技术、材料科学、生物物理学和医学等各个领域。在DNA折纸纳米结构的众多应用中，它们作为通过分子光刻对有机和无机材料进行图案化的掩模受到了极大关注。在这里，我们描述了一种在云母表面自组装有序单层DNA折纸纳米结构的方案，以及随后通过DNA折纸掩模的定向吸附在大面积上制备规则蛋白质图案的方法。虽然图案的几何形状由DNA折纸纳米结构的形状决定，但通过调整吸附缓冲液的蛋白质浓度和阳离子强度，掩模孔内的蛋白质覆盖率可以从单个蛋白质变化到致密单层。
