Thompson Richard B, Fierke Carol A
University of Maryland School of Medicine, Baltimore, MD, United States.
University of Michigan, Ann Arbor, MI, United States.
Methods Enzymol. 2017;589:281-299. doi: 10.1016/bs.mie.2017.01.003. Epub 2017 Mar 9.
Fluorescence-based biosensors have shown themselves to be a powerful tool for the study of a variety of chemical species in biological systems, notably including metal ions. This chapter provides an overview of several important issues in using such sensors to study metallobiochemistry. These issues include selectivity for the analyte over potential interferents, including those that do not themselves induce a signal, the different forms in which metal ions are found (speciation), the utility of metal ion buffers, and the importance of kinetics in studying metal ion binding reactions. Finally, the chapter briefly discusses some of the issues in understanding whole-organism distribution of metal ions and its control.
基于荧光的生物传感器已证明自身是研究生物系统中各种化学物质，尤其是金属离子的有力工具。本章概述了使用此类传感器研究金属生物化学时的几个重要问题。这些问题包括对分析物相对于潜在干扰物（包括那些本身不诱导信号的干扰物）的选择性、金属离子存在的不同形式（形态）、金属离子缓冲剂的效用以及动力学在研究金属离子结合反应中的重要性。最后，本章简要讨论了理解金属离子在整个生物体中的分布及其调控方面的一些问题。
