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制备针对单纯疱疹病毒DNA聚合酶的具有预定特异性的抗体及其在该酶特性鉴定中的应用。

Production of antibodies of predetermined specificity against herpes simplex virus DNA polymerase and their use in characterization of the enzyme.

作者信息

Thomas M S, Banks L M, Purifoy D J, Powell K L

机构信息

Department of Biochemical Virology, Wellcome Research Laboratories, Beckenham, Kent, United Kingdom.

出版信息

J Virol. 1988 May;62(5):1550-7. doi: 10.1128/JVI.62.5.1550-1557.1988.

DOI:10.1128/JVI.62.5.1550-1557.1988
PMID:2833607
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC253181/
Abstract

Peptides from preselected regions of the herpes simplex virus DNA polymerase were used to generate monospecific antisera to defined regions of the enzyme. The antisera were used to localize the polymerase within the infected cell and to determine the time of synthesis during productive infection. Comparison with a neutralizing polyclonal antiserum was used to show the specificity of the peptide antisera. By using the antisera the stabilities of the DNA polymerase, the alkaline nuclease, and the major DNA-binding protein were determined, and the state of phosphorylation of the DNA polymerase was compared with each of these proteins.

摘要

来自单纯疱疹病毒DNA聚合酶预选区域的肽段被用于产生针对该酶特定区域的单特异性抗血清。这些抗血清被用于在受感染细胞内定位聚合酶，并确定生产性感染期间的合成时间。与中和多克隆抗血清进行比较以显示肽抗血清的特异性。通过使用这些抗血清，确定了DNA聚合酶、碱性核酸酶和主要DNA结合蛋白的稳定性，并将DNA聚合酶的磷酸化状态与这些蛋白中的每一种进行了比较。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc04/253181/311f0bbacbdf/jvirol00084-0092-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc04/253181/7b1725d93737/jvirol00084-0087-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc04/253181/dedb89f9b7c4/jvirol00084-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc04/253181/213f4abbb665/jvirol00084-0089-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc04/253181/15b4b88a6f82/jvirol00084-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc04/253181/085ecdedf695/jvirol00084-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc04/253181/f9b9646c019a/jvirol00084-0091-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc04/253181/311f0bbacbdf/jvirol00084-0092-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc04/253181/7b1725d93737/jvirol00084-0087-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc04/253181/dedb89f9b7c4/jvirol00084-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc04/253181/213f4abbb665/jvirol00084-0089-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc04/253181/15b4b88a6f82/jvirol00084-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc04/253181/085ecdedf695/jvirol00084-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc04/253181/f9b9646c019a/jvirol00084-0091-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc04/253181/311f0bbacbdf/jvirol00084-0092-a.jpg

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