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65的全基因组序列和甲基化组分析

Complete Genome Sequence and Methylome Analysis of 65.

作者信息

Fomenkov Alexey, Vincze Tamas, Degtyarev Sergey K, Roberts Richard J

机构信息

New England BioLabs, Ipswich, Massachusetts, USA.

SibEnzyme Ltd., Novosibirsk, Russia.

出版信息

Genome Announc. 2017 Mar 23;5(12):e00060-17. doi: 10.1128/genomeA.00060-17.

DOI:10.1128/genomeA.00060-17
PMID:28336599
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5364224/
Abstract

65 is the original source strain for the restriction enzyme Acc65I. Its complete sequence and full methylome were determined using single-molecule real-time (SMRT) sequencing.

摘要

65是限制性内切酶Acc65I的原始来源菌株。使用单分子实时(SMRT)测序确定了其完整序列和全甲基化组。

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本文引用的文献

1
Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data.非杂交、基于长读长 SMRT 测序数据的完成微生物基因组组装。
Nat Methods. 2013 Jun;10(6):563-9. doi: 10.1038/nmeth.2474. Epub 2013 May 5.
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Going beyond five bases in DNA sequencing.超越 DNA 测序的五个碱基。
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Characterization of DNA methyltransferase specificities using single-molecule, real-time DNA sequencing.利用单分子实时 DNA 测序技术对 DNA 甲基转移酶特异性进行表征。
Nucleic Acids Res. 2012 Feb;40(4):e29. doi: 10.1093/nar/gkr1146. Epub 2011 Dec 7.
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Direct detection of DNA methylation during single-molecule, real-time sequencing.单分子实时测序中 DNA 甲基化的直接检测。
Nat Methods. 2010 Jun;7(6):461-5. doi: 10.1038/nmeth.1459. Epub 2010 May 9.
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REBASE--a database for DNA restriction and modification: enzymes, genes and genomes.REBASE--一个用于 DNA 限制和修饰的数据库:酶、基因和基因组。
Nucleic Acids Res. 2010 Jan;38(Database issue):D234-6. doi: 10.1093/nar/gkp874. Epub 2009 Oct 21.
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Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae.肺炎克雷伯菌中限制性内切酶KpnI的识别序列。
Nucleic Acids Res. 1978 Nov;5(11):4055-64. doi: 10.1093/nar/5.11.4055.