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肺炎克雷伯菌中限制性内切酶KpnI的识别序列。

Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae.

作者信息

Tomassini J, Roychoudhury R, Wu R, Roberts R J

出版信息

Nucleic Acids Res. 1978 Nov;5(11):4055-64. doi: 10.1093/nar/5.11.4055.

DOI:10.1093/nar/5.11.4055
PMID:82935
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342733/
Abstract

We have determined the recognition sequence of the restriction endonuclease KpnI, previously isolated from Klebsiella pneumoniae. The enzyme cleaves the twofold rotationally symmetric sequence (see book for formula) at the positions indicated by the arrows, producing 3' protruding cohesive ends, four nucleotides in length. The specific cleavage site was unambiguously deduced using both 3' and 5' end analyses of KpnI generated restriction fragments of simian-virus 40 (SV40) DNA (1 site), adenovirus-2 (Ad-2) DNA (8 sites), and a plasmid (pCRI) DNA (2 sites).

摘要

我们已经确定了限制性内切酶KpnI的识别序列，该酶先前是从肺炎克雷伯菌中分离出来的。该酶在箭头所示位置切割双重旋转对称序列（公式见本书），产生长度为四个核苷酸的3'突出粘性末端。通过对KpnI产生的猿猴病毒40（SV40）DNA（1个位点）、腺病毒2（Ad-2）DNA（8个位点）和质粒（pCRI）DNA（2个位点）的限制性片段进行3'和5'末端分析，明确推导了特异性切割位点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/374c/342733/9e687afb9d07/nar00472-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/374c/342733/9e687afb9d07/nar00472-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/374c/342733/9e687afb9d07/nar00472-0098-a.jpg

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R.KpnI, an HNH superfamily REase, exhibits differential discrimination at non-canonical sequences in the presence of Ca2+ and Mg2+.R.KpnI是一种HNH超家族限制性内切酶，在存在Ca2+和Mg2+的情况下，对非规范序列表现出不同的识别能力。

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Nucleotide recognition sequence at the cleavage site of Haemophilus aegyptius II (Hae II) restriction endonuclease.埃及嗜血杆菌II（Hae II）限制性内切酶切割位点的核苷酸识别序列。
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