Ladd L, Brandon R, Coulston J, Daniel L, Dimmock C, Lavin M
Department of Biochemistry, University of Queensland, Brisbane, Australia.
Leuk Res. 1988;12(2):143-9. doi: 10.1016/0145-2126(88)90074-4.
Circulating lymphocytes and tumour cells from 12 sheep experimentally infected with bovine leukaemia virus (BLV), for periods of time varying from 9 to 48 weeks, were analysed for evidence of integrated and unintegrated provirus. Hybridization analysis demonstrated that the provirus was integrated at one or two sites in all cases. Integration was observed at different sites in the animals studied and there was no evidence of unintegrated virus molecules in infected sheep lymphocytes or tumour cells. The data obtained support a monoclonal origin of different tumours in the same sheep.
对12只经实验感染牛白血病病毒（BLV）9至48周不等的绵羊的循环淋巴细胞和肿瘤细胞进行分析，以寻找整合型和非整合型前病毒的证据。杂交分析表明，在所有情况下前病毒都整合在一个或两个位点。在所研究的动物中观察到整合发生在不同位点，且在感染绵羊的淋巴细胞或肿瘤细胞中没有非整合病毒分子的证据。所获得的数据支持同一只绵羊体内不同肿瘤的单克隆起源。
