INRA, Univ Pau & Pays de l'Adour, UMR1419 Nutrition Metabolism and Aquaculture, F-64310, Saint Pée sur Nivelle, France.
Sci Rep. 2017 Mar 23;7(1):363. doi: 10.1038/s41598-017-00458-4.
Rainbow trout (Oncorhynchus mykiss) is considered a "glucose-intolerant" species. With the aim of programming trout to improve their metabolic use of dietary carbohydrates, we hypothesised that a hypoxic stimulus applied during embryogenesis could later affect glucose metabolism at the first-feeding stage. An acute hypoxic stimulus (2.5 or 5.0 mg·L O) was applied for 24 h to non-hatched embryos or early hatched alevins followed by a challenge test with a high carbohydrate diet at first-feeding. The effectiveness of the early hypoxic stimulus was confirmed by the induction of oxygen-sensitive markers such as egln3. At first-feeding, trout previously subjected to the 2.5 mg·L O hypoxia displayed a strong induction of glycolytic and glucose transport genes, whereas these glucose metabolism-related genes were affected much less in trout subjected to the less severe (5.0 mg·L O) hypoxia. Our results demonstrate that an acute hypoxic stimulus during early development can affect glucose metabolism in trout at first-feeding.
虹鳟（Oncorhynchus mykiss）被认为是一种“不耐葡萄糖”的物种。为了通过编程使鳟鱼改善其对膳食碳水化合物的代谢利用，我们假设胚胎发生期间应用缺氧刺激以后可以在首次摄食阶段影响葡萄糖代谢。将急性缺氧刺激（2.5 或 5.0 mg·L O）施加于未孵化的胚胎或早期孵化的仔鱼 24 h，随后在首次摄食时用高碳水化合物饮食进行挑战测试。通过诱导氧敏感标记物如 egln3 证实早期缺氧刺激的有效性。在首次摄食时，先前经历 2.5 mg·L O 缺氧的鳟鱼显示出强烈的糖酵解和葡萄糖转运基因诱导，而在经历较低严重程度（5.0 mg·L O）缺氧的鳟鱼中，这些与葡萄糖代谢相关的基因受影响要小得多。我们的研究结果表明，早期发育期间的急性缺氧刺激可以影响首次摄食时鳟鱼的葡萄糖代谢。
