Deptula Paulina, Chamlagain Bhawani, Edelmann Minnamari, Sangsuwan Panchanit, Nyman Tuula A, Savijoki Kirsi, Piironen Vieno, Varmanen Pekka
Department of Food and Environmental Sciences, University of Helsinki Helsinki, Finland.
Proteomics Unit, Institute of Biotechnology, University of Helsinki Helsinki, Finland.
Front Microbiol. 2017 Mar 8;8:368. doi: 10.3389/fmicb.2017.00368. eCollection 2017.
is a traditional dairy bacterium and a producer of short chain fatty acids (propionic and acetic acids) as well as vitamin B12. In food applications, it is a promising organism for fortification with B12 vitamin since it is generally recognized as safe (GRAS) and it is able to synthesize biologically active form of the vitamin. In the present study, vitamin B12 and pseudovitamin biosynthesis by was monitored by UHPLC as a function of growth in food-like conditions using a medium mimicking cheese environment, without cobalt or 5,6-dimethylbenzimidazole (DMBI) supplementation. Parallel growth experiments were performed in industrial-type medium known to support the biosynthesis of vitamin B12. The production of other key metabolites in the two media were determined by HPLC, while the global protein production was compared by gel-based proteomics to assess the effect of growth conditions on the physiological status of the strain and on the synthesis of different forms of vitamin. The results revealed distinct protein and metabolite production, which reflected the growth conditions and the potential of for synthesizing nutritionally relevant amounts of active vitamin B12 regardless of the metabolic state of the cells.
是一种传统的乳制品细菌，也是短链脂肪酸（丙酸和乙酸）以及维生素B12的生产者。在食品应用中，它是一种有前景的用于强化维生素B12的微生物，因为它通常被认为是安全的（GRAS），并且能够合成这种维生素的生物活性形式。在本研究中，在模拟奶酪环境的培养基中，在不添加钴或5,6 - 二甲基苯并咪唑（DMBI）的情况下，通过超高效液相色谱（UHPLC）监测了在类似食品条件下生长过程中维生素B12和假维生素的生物合成。在已知支持维生素B12生物合成的工业型培养基中进行了平行生长实验。通过高效液相色谱（HPLC）测定了两种培养基中其他关键代谢物的产量，同时通过基于凝胶的蛋白质组学比较了全局蛋白质产量，以评估生长条件对菌株生理状态和不同形式维生素合成的影响。结果揭示了不同的蛋白质和代谢物产量，这反映了生长条件以及无论细胞代谢状态如何合成营养相关量活性维生素B12的潜力。
