Piwowarek Kamil, Lipińska Edyta, Hać-Szymańczuk Elżbieta, Bzducha-Wróbel Anna, Synowiec Alicja
Department of Biotechnology, Microbiology and Food Evaluation, Division of Food Biotechnology and Microbiology, Faculty of Food Sciences, Warsaw University of Life Sciences - SGGW (WULS-SGGW), Nowoursynowska 159c Street, 02-776, Warsaw, Poland.
Antonie Van Leeuwenhoek. 2018 Jun;111(6):921-932. doi: 10.1007/s10482-017-0991-7. Epub 2017 Nov 25.
The purpose of this study was to determine the potential for biosynthesis of propionic acid and vitamin B12 by Propionibacterium freudenreichii T82 in a medium containing various sources of carbon (glucose, fructose, and saccharose). These sugars are present in apple pomaces, which are the waste from the production of apple juice. Using statistical analysis design of experiments (DoE), the results allowed us to determine which sugars (carbon sources) exert the most beneficial influence on the biosynthesis of propionic acid and cobalamin. The highest production of propionic acid by the tested bacterial strain was obtained in a medium in which glucose accounted for at least 50% of the available carbon sources. Depending on the culture medium, the concentration of this metabolite ranged from 23 to 40 g/L. P. freudenreichii T82 produced the smallest amount of acid in medium in which the dominant nutrient source was saccharose. The results obtained indicated an inverse relationship between the amount of acid produced by the bacteria and vitamin B12 biosynthesis. Because of the high efficiency of propionic acid biosynthesis by P. freudenreichii T82, the prospect of using this strain to obtain propionate with the simultaneous disposal of waste materials (such as apple pomaces) which contain glucose and/or fructose is very promising.
本研究的目的是确定费氏丙酸杆菌T82在含有各种碳源（葡萄糖、果糖和蔗糖）的培养基中生物合成丙酸和维生素B12的潜力。这些糖类存在于苹果渣中，苹果渣是苹果汁生产过程中的废弃物。通过实验设计的统计分析（DoE），结果使我们能够确定哪些糖类（碳源）对丙酸和钴胺素的生物合成产生最有利的影响。在所测试的细菌菌株中，丙酸产量最高的情况是在葡萄糖占可用碳源至少50%的培养基中获得的。根据培养基的不同，这种代谢物的浓度范围为23至40克/升。在以蔗糖为主导营养源的培养基中，费氏丙酸杆菌T82产生的酸量最少。所得结果表明细菌产生的酸量与维生素B12生物合成之间存在反比关系。由于费氏丙酸杆菌T82生物合成丙酸的效率很高，利用该菌株同时处理含有葡萄糖和/或果糖的废料（如苹果渣）来获得丙酸盐的前景非常广阔。
