Mihailović Vladimir, Katanić Jelena, Mišić Danijela, Stanković Vesna, Mihailović Mirjana, Uskoković Aleksandra, Arambašić Jelena, Solujić Slavica, Mladenović Milan, Stanković Nevena
Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia.
Food Funct. 2014 Aug;5(8):1795-803. doi: 10.1039/c4fo00088a.
The objective of this work was to investigate the effects of the methanol extracts of Gentiana cruciata L. aerial parts (GCA) and roots (GCR) against carbon tetrachloride-induced liver injury in rats. Pretreatment with GCA and GCR, containing sweroside, swertiamarin and gentiopicrin in high concentrations, dose-dependently and significantly decreased the levels of serum transaminases, alkaline phosphatase and total bilirubin, whereas an increase in the level of total protein was found compared with the CCl4-treated group. Moreover, oral administration of extracts significantly enhanced antioxidant enzyme activities (superoxide dismutase and catalase), increased the content of glutathione and decreased the content of TBARS. Microscopic evaluations of the liver revealed CCl4-induced lesions and related toxic manifestations that were minimal in the liver of rats pretreated with extracts at the dose of 400 mg per kg b.w. The results suggest that the use of G. cruciata extracts has a merit as a potent candidate in protecting the liver against chemical induced toxicity.
本研究旨在探讨龙胆地上部分（GCA）和根部（GCR）的甲醇提取物对四氯化碳诱导的大鼠肝损伤的影响。高浓度含獐牙菜苷、獐牙菜苦苷和龙胆苦苷的GCA和GCR预处理，剂量依赖性且显著降低血清转氨酶、碱性磷酸酶和总胆红素水平，而与四氯化碳处理组相比，总蛋白水平有所升高。此外，提取物口服给药显著增强抗氧化酶活性（超氧化物歧化酶和过氧化氢酶），增加谷胱甘肽含量并降低丙二醛含量。肝脏的显微镜评估显示，四氯化碳诱导的损伤及相关毒性表现在用400mg/kg体重剂量提取物预处理的大鼠肝脏中最小。结果表明，使用龙胆提取物作为保护肝脏免受化学诱导毒性的有效候选物具有优势。
