O-Thong Sompong, Khongkliang Peerawat, Mamimin Chonticha, Singkhala Apinya, Prasertsan Poonsuk, Birkeland Nils-Kåre
Research Center in Energy and Environment, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand; Biotechnology Program, Department of Biology, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand.
Biotechnology Program, Department of Biology, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand.
Genom Data. 2017 Feb 28;12:49-51. doi: 10.1016/j.gdata.2017.02.012. eCollection 2017 Jun.
sp. strain PSU-2 was isolated from thermophilic hydrogen producing reactor and subjected to draft genome sequencing on 454 pyrosequencing and annotated on RAST. The draft genome sequence of strain PSU-2 contains 2,552,497 bases with an estimated G + C content of 35.2%, 2555 CDS, 8 rRNAs and 57 tRNAs. The strain had a number of genes responsible for carbohydrates metabolic, amino acids and derivatives, and protein metabolism of 17.7%, 14.39% and 9.81%, respectively. Strain PSU-2 also had gene responsible for hydrogen biosynthesis as well as the genes related to Ni-Fe hydrogenase. Comparative genomic analysis indicates strain PSU-2 shares about 94% genome sequence similarity with LX-11. The nucleotide sequence of this draft genome was deposited into DDBJ/ENA/GenBank under the accession MSQD00000000.
菌株PSU - 2从嗜热产氢反应器中分离出来,并在454焦磷酸测序平台上进行草图基因组测序,随后在RAST上进行注释。菌株PSU - 2的草图基因组序列包含2,552,497个碱基,估计G + C含量为35.2%,有2555个编码序列(CDS)、8个rRNA和57个tRNA。该菌株分别有17.7%、14.39%和9.81%的基因负责碳水化合物代谢、氨基酸及其衍生物代谢以及蛋白质代谢。菌株PSU - 2还具有负责氢气生物合成的基因以及与镍铁氢化酶相关的基因。比较基因组分析表明,菌株PSU - 2与LX - 11的基因组序列相似度约为94%。该草图基因组的核苷酸序列已保存在DDBJ/ENA/GenBank中,登录号为MSQD00000000。