Liu Kun, He Le, Li Shiwei, Tian Fengshou, Sun Zhongke, Li Chengwei
1College of Life Sciences and Agronomy, Zhoukou Normal University, Room 407, Life Science Building, East Wenchang Road, Chuanhui District, Zhoukou, 466001 China.
3Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou, 466001 China.
3 Biotech. 2018 Feb;8(2):85. doi: 10.1007/s13205-017-1042-9. Epub 2018 Jan 19.
A high-quality draft genome sequence of a multi-antibiotic resistant strain, strain LK1 isolated from the rhizosphere of is described. Strain LK1 produced several types of phytohormones, which included Indole-3-acetic acid (IAA) and Zeatin. Phylogenetic analysis of the gene of strain LK1 showed a close relationship to and (99% gene sequence similarity). The genome size of strain LK1 was estimated to be 7.16 Mb that consisted of 82 contigs contained in 49 scaffolds. The G + C content of the genome was 45.63% and it encoded 6499 genes, 115 pseudo genes, 8 rRNAs, 33 tRNAs, and 4 ncRNAs, based on the Prokaryotic Genome Annotation Pipeline (PGAP). Further analysis of the genome of strain LK1 identified 16 genes encoding monooxygenase and six genes encoding cytochrome P450, which may be potentially involved in zeatin biosynthesis. The draft whole genome sequence and annotation of strain LK1 has been deposited at DDBJ/EMBL/GenBank under the accession number (PEII00000000).
本文描述了从[植物名称]根际分离出的一株多重抗生素抗性菌株LK1的高质量基因组草图序列。菌株LK1能产生多种植物激素，包括吲哚 - 3 - 乙酸（IAA）和玉米素。对菌株LK1的[基因名称]基因进行系统发育分析表明，它与[其他菌株名称1]和[其他菌株名称2]关系密切（基因序列相似度为99%）。菌株LK1的基因组大小估计为7.16 Mb，由49个支架中的82个重叠群组成。根据原核生物基因组注释管道（PGAP），该基因组的G + C含量为45.63%，编码6499个基因、115个假基因、8个rRNA、33个tRNA和4个非编码RNA。对菌株LK1基因组的进一步分析确定了16个编码单加氧酶的基因和6个编码细胞色素P450的基因，它们可能潜在参与玉米素的生物合成。菌株LK1的全基因组草图序列及注释已保存在DDBJ/EMBL/GenBank，登录号为（PEII00000000）。
