Liu Jie, Li Junlian, Liu Jiao, Zhao Xiuqin, Lian Lulu, Liu Haican, Lu Bing, Yu Qin, Zhang Jingrui, Qi Yingcheng, Wan Kanglin
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China; Chaoyang Center for Disease Control and Prevention, Beijing, 100021, China.
The Chest Hospital of Xinjiang Uyghur Autonomous Region, Ürümqi 830001, China.
Biomed Res Int. 2017;2017:3179535. doi: 10.1155/2017/3179535. Epub 2017 Feb 28.
We studied the genetic diversity of clinical isolates from patients with tuberculosis in the multiethnic area of Xinjiang autonomous region in China. A total of 311 clinical isolates were collected in 2006 and 2011 and genotyped by two genotyping methods. All isolates were grouped into 68 distinct spoligotypes using the spoligotyping method. The Beijing family was dominant, followed by T1 and CAS. MIRU-VNTR results showed that a total of 195 different VNTR types were identified. Ten of the 15 loci were highly or moderately discriminant according to their HGDI scores, and 13 loci had good discriminatory power in non-Beijing family strains, whereas only two loci had good discriminatory power in Beijing family strains. Chi-square tests demonstrated that there were no correlations between four characteristics (sex, age, type of case, and treatment history) and the Beijing family. In summary, Beijing family strains were predominant in Xinjiang, and the VNTR-15 locus-set was suitable for genotyping all Xinjiang strains, but not for the Beijing family strains. Thus, these data suggested that different genotype distributions may exist in different regions; MLVA locus-sets should be adjusted accordingly, with newly added loci to increase resolution if necessary.
我们研究了中国新疆自治区多民族地区结核病患者临床分离株的遗传多样性。2006年和2011年共收集了311株临床分离株，并采用两种基因分型方法进行基因分型。使用 spoligotyping 方法将所有分离株分为68种不同的 spoligotypes。北京家族占主导地位，其次是T1和CAS。MIRU-VNTR结果显示，共鉴定出195种不同的VNTR类型。根据HGDI评分，15个位点中的10个具有高或中等的鉴别能力，13个位点在非北京家族菌株中具有良好的鉴别能力，而在北京家族菌株中只有两个位点具有良好的鉴别能力。卡方检验表明，四个特征（性别、年龄、病例类型和治疗史）与北京家族之间没有相关性。总之，北京家族菌株在新疆占主导地位，VNTR-15位点集适用于所有新疆菌株的基因分型，但不适用于北京家族菌株。因此，这些数据表明不同地区可能存在不同的基因型分布；应相应调整MLVA位点集，如有必要可添加新的位点以提高分辨率。
