Gowane G R, Akram Najif, Prince L L L, Prakash Ved, Kumar Arun
ICAR-Central Sheep & Wool Research Institute, Avikanagar via, Jaipur, Rajasthan, 304501, India.
Trop Anim Health Prod. 2017 Apr;49(4):867-873. doi: 10.1007/s11250-017-1277-9. Epub 2017 Mar 23.
Enterotoxaemia (ET) is a fatal enteric disease of small ruminants attributable to a toxigenic type of Clostridium perfringens. The key strategy for prevention of ET is the management and vaccination. Present study aimed at identifying the sources of variation for ET vaccine response especially against epsilon toxin in 173 sheep that included 83 Avikalin and 90 Malpura lambs raised at the institute flock in the semi-arid region of India. The mean age at vaccination was 90 days. Sera were tested by blocking ELISA. Study showed significant variability for response to ET vaccine. 5.2% animals had + positivity, 20.8% animals had ++ positivity, 51.4% animals had +++ positivity and 22.5% animals had ++++ positivity. Amongst environmental determinants, breed, season, sex and age at vaccination proved to be non-significant sources of variation (P > 0.05). MHC genotypes with DRB1 gene and DQA2 genes also revealed non-significant association with ET vaccine response; however, a trend of decreasing PI values with increasing ranks was observed. Study revealed strong response of epsilon toxin along with complexity of the ET vaccine response as phenotype to be explained by genetic and non-genetic factors. The importance of better management practices and vaccination is suggested for preventive measures.
肠毒血症（ET）是一种由产气荚膜梭菌产毒型引起的小反刍动物致命性肠道疾病。预防ET的关键策略是管理和疫苗接种。本研究旨在确定173只绵羊ET疫苗反应尤其是对ε毒素反应的变异来源，这些绵羊包括在印度半干旱地区研究所羊群中饲养的83只阿维卡林羊和90只马尔普拉羊。接种疫苗时的平均年龄为90天。通过阻断ELISA检测血清。研究表明，ET疫苗反应存在显著变异性。5.2%的动物为 + 阳性，20.8%的动物为 ++ 阳性，51.4%的动物为 +++ 阳性，22.5%的动物为 ++++ 阳性。在环境决定因素中，品种、季节、性别和接种疫苗时的年龄被证明不是显著的变异来源（P > 0.05）。具有DRB1基因和DQA2基因的MHC基因型也显示与ET疫苗反应无显著关联；然而，观察到PI值随等级增加而降低的趋势。研究揭示了ε毒素的强烈反应以及ET疫苗反应作为一种表型的复杂性，其可由遗传和非遗传因素来解释。建议采取更好的管理措施和疫苗接种作为预防措施。
