Jian Rui, Wang Shi-Wei, Zhang Wei-Xin, Zhang Lu-Ping
Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang, 050024, Hebei, People's Republic of China.
College of Animal Science, Tarim University, Alar, 843300, China.
Syst Parasitol. 2017 May;94(4):511-525. doi: 10.1007/s11230-017-9714-8. Epub 2017 Mar 23.
Habronematid nematodes were collected from the stomachs of donkeys, Equus asinus L., in the Tarim Basin, Xinjiang, China. After examination by light and scanning electron microscopy, Habronema muscae (Carter, 1861) and H. majus (Creplin, 1849) were identified. The morphology of our specimens representing H. muscae (Carter, 1861) agreed well with previous redescriptions in the shape of the lateral lips, origin of the lateral alae, ratio of left and right spicules, and number and arrangement of caudal papillae. However, H. majus (Creplin, 1849) differs from H. microstoma (Schneider, 1866) in the arrangement of the caudal papillae in the male. Moreover, molecular analysis also showed interspecific differences of 26.2-28.2% in ITS2 and 8.6-8.9% in cox1 between H. majus and H. microstoma, a divergence much higher than the known intraspecific variation of Habronema spp. (6.6-8.7% in ITS2; 0.2-2.2% in cox1). The results indicate that both H. microstoma (Schneider, 1866) and H. majus (Creplin, 1849) are valid species.
在中国新疆塔里木盆地，从家驴（Equus asinus L.）的胃中采集到柔线虫科线虫。经光学显微镜和扫描电子显微镜检查后，鉴定出蝇柔线虫（Habronema muscae，Carter，1861）和大型柔线虫（H. majus，Creplin，1849）。我们所鉴定为蝇柔线虫（Carter，1861）的标本形态，在侧唇形状、侧翼起始部位、左右交合刺比例以及尾乳突数量和排列方式等方面，与之前的重新描述非常吻合。然而，大型柔线虫（Creplin，1849）在雄虫尾乳突排列方式上与小口柔线虫（Schneider，1866）不同。此外，分子分析还显示，大型柔线虫和小口柔线虫之间的ITS2种间差异为26.2 - 28.2%，cox1种间差异为8.6 - 8.9%，这种差异远高于柔线虫属已知的种内变异（ITS2为6.6 - 8.7%；cox1为0.2 - 2.2%）。结果表明，小口柔线虫（Schneider，1866）和大型柔线虫（Creplin，1849）均为有效物种。
