College of Environmental Science and Engineering, Fujian Normal University, Fuzhou, Fujian, 350007, China.
College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou, Fujian, 350007, China.
Sci Rep. 2017 Mar 24;7:45343. doi: 10.1038/srep45343.
A portable, affordable and simple detector is requested in a "Point-of-Care-Testing" (POCT) system. In this study, we exploited the potentialities of Differential Pressure Gauge (DPG) to the orientation of POCT technology. Alpha fetoprotein (AFP) was chosen as a model analyte that could specifically recognized by its antigen, and a tiny outfits equipped with a DPG was employed as the signal readout. Pt/SiO nanospheres were synthesized and modified with the detection antibody. In the presence of target, a sandwich of immunocomplex specifically formed and the Pt/SiO had been modified on the capture antibody. Which then can be dissolved to release plenty of Pt and the suspensions were transferred into a closed vial filled with appropriated amount of hydrogen peroxide. Subsequently, hydrogen peroxide was decomposed to produce oxygen, resulting in the enhancement of pressure in the closed vial and which can be detected by DPG easily. Under the optimized conditions, the read out signal from DPG had a direct relationship with AFP concentrations in the range of 10~200 ng/mL, and the detection limit was as low as 3.4 ng/mL. The proposed portable sensor had been successfully applied to detect AFP in serum samples with satisfactory results. This strategy holds a great promising in biological analysis as its convenient operations, reliable results and flexible apparatus.
在“即时检验”（POCT）系统中需要一种便携式、经济实惠且简单的探测器。在本研究中，我们利用差压计（DPG）的潜力将 POCT 技术定向化。甲胎蛋白（AFP）被选为模型分析物，其抗原可特异性识别，并且配备有 DPG 的微小设备被用作信号读出。Pt/SiO 纳米球被合成并修饰有检测抗体。在存在靶标时，免疫复合物特异性形成，Pt/SiO 已被修饰在捕获抗体上。然后可以将其溶解以释放大量的 Pt，并且将悬浮液转移到充满适量过氧化氢的封闭小瓶中。随后，过氧化氢分解产生氧气，导致封闭小瓶中的压力增强，并且可以通过 DPG 轻松检测到。在优化条件下，从 DPG 读取的信号与 AFP 浓度在 10-200ng/mL 范围内呈直接关系，检测限低至 3.4ng/mL。所提出的便携式传感器已成功应用于血清样品中 AFP 的检测，结果令人满意。该策略在生物分析中具有很大的应用前景，因为其操作方便、结果可靠且设备灵活。
