Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
School of Future Technology, University of Chinese Academy of Sciences, Beijing, 100049, China.
Adv Healthc Mater. 2017 Jun;6(12). doi: 10.1002/adhm.201601431. Epub 2017 Mar 24.
Clinical applications of current photodynamic therapy (PDT) photosensitizers (PSs) are often limited by their absorption in the UV-vis range that possesses limited tissue penetration ability, leading to ineffective therapeutic response for deep-seated tumors. Alternatively, two-photon excited PS (TPE-PS) using NIR light triggered is one the most promising candidates for PDT improvement. Herein, multimodal polymer nanoparticles (PNPs) from polythiophene derivative as two-photon fluorescence imaging as well as two-photon-excited PDT agent are developed. The prepared PNPs exhibit excellent water dispersibility, high photostability and pH stability, strong fluorescence brightness, and low dark toxicity. More importantly, the PNPs also possess other outstanding features including: (1) the high O quantum yield; (2) the strong two-photon-induced fluorescence and efficient O generation; (3) the specific accumulation in lysosomes of HeLa cells; and (4) the imaging detection depth up to 2100 µm in the mock tissue under two-photon. The multifunctional PNPs are promising candidates as TPE-PDT agent for simultaneous cellular, deep-tissue imaging, and highly efficient in vivo PDT of cancer.
当前光动力疗法（PDT）光敏剂（PS）的临床应用通常受到其在具有有限组织穿透能力的 UV-vis 范围内吸收的限制，导致深部肿瘤的治疗反应无效。另一方面，使用近红外光触发的双光子激发 PS（TPE-PS）是 PDT 改善的最有前途的候选者之一。在此，开发了一种基于聚噻吩衍生物的多模式聚合物纳米粒子（PNP），用作双光子荧光成像以及双光子激发 PDT 剂。所制备的 PNP 表现出优异的水分散性、高光稳定性和 pH 稳定性、强荧光亮度和低暗毒性。更重要的是，PNP 还具有其他出色的特性，包括：（1）高 O 量子产率；（2）强双光子诱导荧光和有效的 O 生成；（3）在 HeLa 细胞的溶酶体中的特异性积累；以及（4）在模拟组织中在双光子下高达 2100 µm 的成像检测深度。多功能 PNP 有望成为 TPE-PDT 剂的候选物，用于同时进行细胞、深层组织成像和高效的体内癌症 PDT。
