Beijing National Laboratory for Molecular Sciences (BNLMS), CAS, Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, 100190, Beijing, China.
University of Chinese Academy of Sciences, 100049, Beijing, China.
Angew Chem Int Ed Engl. 2023 Aug 14;62(33):e202308019. doi: 10.1002/anie.202308019. Epub 2023 Jul 6.
Two-photon-excited photodynamic therapy (TPE-PDT) has significant advantages over conventional photodynamic therapy (PDT). However, obtaining easily accessible TPE photosensitizers (PSs) with high efficiency remains a challenge. Herein, we demonstrate that emodin (Emo), a natural anthraquinone (NA) derivative, is a promising TPE PS with a large two-photon absorption cross-section (TPAC: 380.9 GM) and high singlet oxygen ( O ) quantum yield (31.9 %). When co-assembled with human serum albumin (HSA), the formed Emo/HSA nanoparticles (E/H NPs) possess a giant TPAC (4.02×10 GM) and desirable O generation capability, thus showing outstanding TPE-PDT properties against cancer cells. In vivo experiments reveal that E/H NPs exhibit improved retention time in tumors and can ablate tumors at an ultra-low dosage (0.2 mg/kg) under an 800 nm femtosecond pulsed laser irradiation. This work is beneficial for the use of natural extracts NAs for high-efficiency TPE-PDT.
双光子激发光动力疗法（TPE-PDT）相较于传统光动力疗法（PDT）具有显著优势。然而，获得高效的易得双光子激发光敏剂（PS）仍然是一个挑战。在此，我们证明大黄素（Emo），一种天然蒽醌（NA）衍生物，是一种很有前途的 TPE PS，具有较大的双光子吸收截面（TPAC：380.9 GM）和高单线态氧（ 1 O 2 ）量子产率（31.9%）。当与人血清白蛋白（HSA）共组装时，形成的大黄素/HSA 纳米粒子（E/H NPs）具有巨大的 TPAC（4.02×10 GM）和理想的 1 O 2 生成能力，因此对癌细胞具有出色的 TPE-PDT 性能。体内实验表明，E/H NPs 在肿瘤中有更好的保留时间，并且可以在 800nm 飞秒脉冲激光照射下以超低剂量（0.2mg/kg）消融肿瘤。这项工作有利于利用天然提取物 NAs 进行高效的 TPE-PDT。
