Kucharewicz Weronika, Distelfeld Assaf, Bilger Wolfgang, Müller Maren, Munné-Bosch Sergi, Hensel Götz, Krupinska Karin
Institute of Botany, Christian-Albrechts-University of Kiel, Kiel, Germany.
Department of Molecular Biology and Ecology of Plants, University of Tel Aviv, Tel Aviv, Israel.
J Exp Bot. 2017 Feb 1;68(5):983-996. doi: 10.1093/jxb/erw501.
WHIRLY1 in barley was isolated as a potential regulator of the senescence-associated gene HvS40. In order to investigate whether the plastid-nucleus-located DNA/RNA-binding protein WHIRLY1 plays a role in regulation of leaf senescence, primary foliage leaves from transgenic barley plants with an RNAi-mediated knockdown of the WHIRLY1 gene were characterized by typical senescence parameters, namely pigment contents, function and composition of the photosynthetic apparatus, as well as expression of selected genes known to be either down- or up-regulated during leaf senescence. When the plants were grown at low light intensity, senescence progression was similar between wild-type and RNAi-W1 plants. Likewise, dark-induced senescence of detached leaves was not affected by reduction of WHIRLY1. When plants were grown at high light intensity, however, senescence was induced prematurely in wild-type plants but was delayed in RNAi-W1 plants. This result suggests that WHIRLY1 plays a role in light sensing and/or stress communication between chloroplasts and the nucleus.
大麦中的WHIRLY1作为衰老相关基因HvS40的潜在调节因子被分离出来。为了研究位于质体-细胞核的DNA/RNA结合蛋白WHIRLY1是否在叶片衰老调控中发挥作用，对通过RNA干扰介导敲低WHIRLY1基因的转基因大麦植株的初生叶片进行了典型衰老参数的表征，即色素含量、光合机构的功能和组成，以及已知在叶片衰老过程中下调或上调的特定基因的表达。当植株在低光照强度下生长时，野生型和RNAi-W1植株的衰老进程相似。同样，离体叶片的暗诱导衰老不受WHIRLY1减少的影响。然而，当植株在高光照强度下生长时，野生型植株的衰老提前诱导，但RNAi-W1植株的衰老延迟。这一结果表明，WHIRLY1在叶绿体与细胞核之间的光感知和/或应激信号传递中发挥作用。
