Institute of Botany, Christian-Albrechts-University, Kiel, Germany.
Central Microscopy, Department of Biology, Christian-Albrechts-University, Kiel, Germany.
Planta. 2022 Mar 13;255(4):84. doi: 10.1007/s00425-022-03854-x.
In accordance with a key role of WHIRLY1 in light-acclimation mechanisms, typical features of acclimation to high light, including photosynthesis and leaf morphology, are compromised in WHIRLY1 deficient plants. Acclimation to the environment requires efficient communication between chloroplasts and the nucleus. Previous studies indicated that the plastid-nucleus located WHIRLY1 protein is required for the communication between plastids and the nucleus in situations of high light exposure. To investigate the consequences of WHIRLY1 deficiency on the light acclimation of photosynthesis and leaf anatomy, transgenic barley plants with an RNAi-mediated knockdown of HvWHIRLY1 were compared to wild-type plants when growing at low and high irradiance. While wild-type plants showed the typical light acclimation responses, i.e. higher photosynthetic capacity and thicker leaves, the WHIRLY1 deficient plants were not able to respond to differences in irradiance. The results revealed a systemic role of WHIRLY1 in light acclimation by coordinating responses at the level of the chloroplast and the level of leaf morphology.
根据 WHIRLY1 在光适应机制中的关键作用，WHIRLY1 缺失的植物在光合作用和叶片形态等适应高光的典型特征上受到了损害。适应环境需要叶绿体和细胞核之间进行有效的通讯。先前的研究表明，定位于质体-细胞核的 WHIRLY1 蛋白在高光暴露情况下，对于质体和细胞核之间的通讯是必需的。为了研究 WHIRLY1 缺失对光合作用和叶片解剖结构光适应的影响，将 RNAi 介导的 HvWHIRLY1 敲低的转基因大麦植株与在低光照和高光照下生长的野生型植株进行了比较。虽然野生型植株表现出典型的光适应反应，即更高的光合能力和更厚的叶片，但 WHIRLY1 缺失的植株无法对光照强度的差异做出反应。结果表明，WHIRLY1 通过协调叶绿体和叶片形态水平的响应，在光适应中发挥系统作用。
