Ino T, Hirano M, Kurosawa Y
Institute for Comprehensive Medical Science, Fujita-Gakuen Health University, School of Medicine, Aichi, Japan.
Biochem Biophys Res Commun. 1988 Apr 15;152(1):257-63. doi: 10.1016/s0006-291x(88)80708-3.
In a previous paper (Proc. Natl. Acad. Sci. USA 84: 4264, 1987) we reported an unusual DNA rearrangement in T-cell receptor beta chain gene loci in cells from a patient with human T-cell leukemia. A D beta 1-J beta 2.3 junction was found on one chromosome, while the other chromosome kept the germline configuration. Although the DNA fragment located between the D beta 1 and J beta 2.3 loci should have disappeared from the cells, it was found on chromosome 6 as an inserted segment. We have now determined the nucleotide sequences bordering both sides of the inserted segment. The signal sequence for D beta-J beta rearrangement at the 5' side of J beta 1.2 gene seems to have been used for the insertion. The 3' end of the inserted segment corresponded to the edge of the signal heptamer at the 5' side of J beta 2.3 which was used for the initial D beta 1-J beta 2.3 joining. This indicates that, during D beta-J beta rearrangement, the intervening sequence was excised as a linear molecule.
在之前的一篇论文（《美国国家科学院院刊》84: 4264, 1987）中，我们报道了一名人类T细胞白血病患者细胞中T细胞受体β链基因座存在异常的DNA重排。在一条染色体上发现了一个Dβ1-Jβ2.3连接，而另一条染色体保持种系构型。尽管位于Dβ1和Jβ2.3基因座之间的DNA片段本应从细胞中消失，但在6号染色体上却作为插入片段被发现。我们现在已经确定了插入片段两侧的核苷酸序列。Jβ1.2基因5'端Dβ-Jβ重排的信号序列似乎被用于插入。插入片段的3'端对应于Jβ2.3基因5'端信号七聚体的边缘，该信号七聚体用于最初的Dβ1-Jβ2.3连接。这表明，在Dβ-Jβ重排过程中，中间序列作为线性分子被切除。
