通过对外部包膜糖蛋白进行寡肽分析快速评估HIV分离株之间的关系。

Rapid assessment of relationships among HIV isolates by oligopeptide analyses of external envelope glycoproteins.

作者信息

Robey W G, Nara P L, Poore C M, Popovic M, McLane M F, Barin F, Essex M, Fischinger P J

机构信息

Abbott Laboratories, Abbott Park, IL 60064.

出版信息

AIDS Res Hum Retroviruses. 1987;3(4):401-8. doi: 10.1089/aid.1987.3.401.

DOI:10.1089/aid.1987.3.401

PMID:2833916

Abstract

The most variable proteins, the gp120's, of the many isolates of HIV-I can be readily compared by two-dimensional oligopeptide maps. The gp120 in a given cell line is completely stable, but the cell line defines the actual gp120 size and may induce minor peptide changes. HTLV-IIIB and LAV differ slightly from each other even when grown in the same cell line, while LAV grown in a B cell line is less related. Molecularly distant isolates have unique patterns. While anti-HTLV-IIIB gp120 antibody neutralized both HTLV-IIIB and LAV, it recognizes only the homologous HTLV-IIIB infected cells in cytotoxicity assays. Structural analysis of isolates should be helpful in defining the range of immunological reactivities among variants as a contribution to a rational approach to a vaccine against AIDS.

摘要

通过二维寡肽图谱可以很容易地比较多种HIV-1分离株中变化最大的蛋白质——gp120。给定细胞系中的gp120是完全稳定的，但细胞系决定了实际的gp120大小，并且可能会引起小的肽段变化。即使在同一细胞系中培养，HTLV-IIIB和LAV彼此之间也略有不同，而在B细胞系中培养的LAV相关性更低。分子距离较远的分离株具有独特的图谱。虽然抗HTLV-IIIB gp120抗体能中和HTLV-IIIB和LAV，但在细胞毒性试验中，它仅识别同源的HTLV-IIIB感染细胞。对分离株进行结构分析，有助于确定变异体之间免疫反应性的范围，从而为研制艾滋病疫苗提供合理的方法。