Akhrem A A, Andreiuk G M, Gurinovich N A, Kisel' M A, Kiselev P A
Biokhimiia. 1987 Dec;52(12):2015-21.
The interaction of fatty acids (FA) with oxyhemoglobin (HbO2) was investigated. It was found that under effect of FA HbO2 is converted into the oxidized low-spin form, i. e., hemichrome. An increase in the level of reactive oxygen species was registered by the ESP method, using the spin traps, 1.2-dioxybenzo-3.5-disulphonate (tiron) and 5.5-dimethylpyrroline-1-oxide (DMPO). To investigate the nature of radical particles, the OH radical traps. thiourea and D-mannitol, as well as superoxide dismutase (SOD) and catalase were employed. The mechanism of interaction of HbO2 and FA is discussed; its feasible physiological role in the functioning of erythrocytes is postulated.
研究了脂肪酸（FA）与氧合血红蛋白（HbO2）的相互作用。结果发现，在FA的作用下，HbO2转化为氧化型低自旋形式，即高铁血红蛋白。使用自旋捕获剂1,2 - 二羟基苯 - 3,5 - 二磺酸盐（钛铁试剂）和5,5 - 二甲基吡咯啉 - 1 - 氧化物（DMPO），通过电子自旋共振（ESP）方法测定了活性氧物种水平的增加。为了研究自由基粒子的性质，使用了羟基自由基捕获剂硫脲和D - 甘露醇，以及超氧化物歧化酶（SOD）和过氧化氢酶。讨论了HbO2与FA的相互作用机制；推测了其在红细胞功能中的可能生理作用。
