Akhrem A A, Andreiuk G M, Kisel' M A, Kurbako V Z, Kiselev P A
Biokhimiia. 1987 Nov;52(11):1927-34.
The interaction of fatty acids (FA) with methemoglobin (met-Hb) was investigated. It was found that under the action of FA met-Hb is rapidly converted into a low-spin form (the so-called hemichrome). This process is reversible as proved by the addition of bovine serum albumin into the system. Using differential spectrophotometry, the efficiency of FA action on met-Hb depending on concentration and nature of FA was estimated. Using the circular dichroism (CD) method, it was demonstrated that under the action of FA the concentration of R-conformation in the met-Hb molecule is increased. The stoichiometry of the hemichrome complex with FA at various FA concentrations was assessed. A comparative analysis of the effect of various detergents with known structures on met-Hb was carried out. The mechanism of met-Hb conversion into hemichrome is discussed.
研究了脂肪酸（FA）与高铁血红蛋白（met-Hb）的相互作用。结果发现，在FA的作用下，met-Hb迅速转化为低自旋形式（即所谓的高铁血色原）。通过向系统中添加牛血清白蛋白证明该过程是可逆的。使用差示分光光度法，评估了FA对met-Hb的作用效率，该效率取决于FA的浓度和性质。使用圆二色性（CD）方法表明，在FA的作用下，met-Hb分子中R-构象的浓度增加。评估了在不同FA浓度下高铁血色原与FA复合物的化学计量。对具有已知结构的各种去污剂对met-Hb的作用进行了比较分析。讨论了met-Hb转化为高铁血色原的机制。
